GGACTGAACATATGTTCCGGGGAGAGTGGCTGCGATTTCGCGTCGGTAAAAATAGCAAATACTCGTTAATGTGCTGTGGG AACGCTTCCTCCCCGGCCCCAAAGTGGCCCCGAAGAAAGTGAGCAAATGTGCGCGCCGCAAGATAGTCGCCGCCGAACAA ACGATAGTGACGAAATGATTTAATTCAACTACCAGCACTCCCGCAAATACGATGAGTATGTCGCGCGGCGGCAACACAAC TCTGGACTTGCAGCCGCTCCTGGCGGAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATC GGTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTCGGCGTTGTTCGTCAAGAAG ATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGATTACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTT CCCCTCGTTCCTGTTCCTCAGCCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGG TGAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATTTCTGGGAAACATGATGTTT GGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGCAGCCCTACGACGCTTCTCTATCCTGATGACCATGCTGCT GGAGCTCAAGATCCTGGGACTGCGACCTTCGAATGCGGTTCAGGTCAGCGTATACGCAATGATCGGTGGAGCGCTGCTGG CCGCCTCTGATGATCTGTCCTTCAACATGAGGGGCTACATCTATGTGATGATCACTAACGCCTTGACCGCCTCGAATGGC GTATATGTGAAGAAAAAACTCGACACCTCGGAGATCGGAAAGTACGGCCTAATGTACTACAACTCGCTGTTTATGTTTCT GCCTGCCCTGGCCCTCAACTATGTTACAGGGAATCTAGATCAGGCGCTGAACTTTGAACAATGGAATGACTCAGTGTTTG TGGTGCAGTTCCTGCTCAGTTGCGTTATGGGTTTCATCCTATCGTACAGCACCATCCTGTGCACGCAATTCAACTCGGCG CTGACCACCACCATTGTGGGATGCCTGAAAAACATCTGCGTAACATATCTGGGCATGTTCATTGGAGGCGACTACGTCTT CTCGTGGCTCAACTGTATTGGGATCAACATCAGCGTGCTGGCTAGTCTGCTCTACACGTACGTCACTTTTCGGCGGAAGC GGGCTCCCGATAAGCAGGACCACTTGCCCAGCACCCGCGGCGAGAATGTCTAGCTTCATCTTAGCTCAAACTCAAGCCAA AGCCATACGATATGTTAAGTGTATTCCGTACTGTGTACTTCAATTGCGATCTGCGATTCGCGACAACGTTCGTATTTGAT TTTCCTACAACCACACAGCGCATCGTCTCTGTACATATATCTAGCTATATTTCTATAAAAATGTAATTTAGCAATTATGT AATTTTAATCACTTTGTACATTTGTCTGCATGCAACTGTTTCCTGCATCGAAAGTGTCATAAGAAATAGAAATACACAGG CTTCCACTCCTCCGCCTACGGAATGGGTGGGCCAGATAAACAATTGTGTTCCGTCTTCTGTTCCATTTTGGGTCATAATT GATAAGATTGCCTGCTCCGATAAGGCATACAACTCGGACGTTGCAAATTAAGCAGACATCTTGCTCTCTCTCACGTGCTT TTCTAATTACTTTTTTGTGTAAAATAAGTAATGTAGTGGAACTGCGTTGTGGGATTTGCCAATTACTTGTGCTTAATAGG TTGTTTATCTATTCTGAGCAAACACGTTGGCGCCTTGAGATTAATACAAAACAAAGTACTGCATTGCCGGACAATCCAGC AGCGTCATCACTCAACATCACTATGTAAAAAGCTTTGGATAGGTTGCTCGATTACTGATTACTCTATATCAAAATTTTGG GTTTTTATGTTGAATAAAAAAAAAACAATATTATCAGCCAAGAACGTGATTGCGAGTGGTGATAGTATTTTTTAGAATAG TACGCCTTCCGATATTTTGTGTTATTTTCCGTTTCATTTGGTAATTGCCTCAAAAGGCTTTTGTTTAGATTGTAGTGCTG AAGAATCTTTTTCTTTGTCTTAAAAGTAAATAAATTTGCGCAGCTGAAACTGTTTTGTTTTAGCGAAATAAGTATGTATA CTTTAAGATGGCTATCTATTTTCTTAGAATTAGATTTTACTTGGTAACTGCCTAGTTCCTTATCAAAAGATTAAGATTTA AATATCCCACATATATGTTGCTAAACTTATCTTTTAAATGGTGACTACTCATTTATCCAAAGAAACAACAATGACGACAT TGAATCGGGTATAAAGCGGCAGAATGAGGTGCTAGTGCCCCGGCAGCACTTTGTTTGGGTTATTTTGGGAGCCCGCAATA TATGATGGGCGGTGGTGAAGTGAGGGATTCGGAGTGGGGACAGGTAATGAGGAAGCCGAAACAGGTTTGCACAGACTTAG CCGTGCGGTGAGGTAAATTGTTAGTCAGCATCAGCCCCGACCGCGTACCCACATTATGGACAAATTGTTTGCTCATATTC GCCGCTATTGAAAGTGGGCACGTACATACGTACGTATGGCTCGAGGGACCAGCTTTAACCGAAGACAGGTAGTCAGGGTC ATGTTTGTGTGCGACGATGATGATGATGATAATAATAATGTAATGATGATGATGCCGACGATCGTGCTGATAAGGTCACC ACCGGGTGCACCTCCCCATATCTCCTCTATGAATCCACAGATCATCATGATGCAACGCTGTTTGCGTCTCCAAAAGCCGC TGGCCCTACGACGAGGCCTTCGCTTGGCCCAGGCTAATAGCCAAGCGGTGGCCACAGAAGCACCCGAGGCGGAACCTCTG GATGCCTTCGAGCGTCAGTACCTCAAGGAGCGAATCGAGATATCGCCCTTCCAGCGGCTGTTCCTGGGGGCAGGATCCTC CATTGCCGCGCTCTTGAATCCAAGACGTCATGACATGATCGCCTGTTTGGGTGAGACGACTGGAGAGGATGCTCTATGGA CAATTTTGGACACCATGCAAGCCAGCGAGGAGGGTCAGCGCATCATGGCTGATAAGCCCCGCATCCACACCAGCACCATT GACTTCAAGTATCTGGAAACTTTGCCACCGGATACCTTTGGAGCCGCTTACGTAAAGTTTCTGAAAGATAATCAAGTCAC GCCGGACAGTCGCATGGCCGTGCGGTTTTTGGAGGACCCGAAGTTGGCCTACTTGATGACGCGATATCGCGAATGCCACG ACCTAATCCACACGGTGCTGGACATGCCCACCAATATGCTGGGCGAGGTGGCTGTCAAGTGGGTGGAGGCTCTGAACACG GGCCTGCCCATGTGCTACGGTGGTGCGGTATTCGGAGCAGTTCGCCTACGTCCCAAGCAGCGGCGTGCGTACTTGAAGCA TTACTTGCCGTGGGCCCTGGAGAACGGCAAGCGGACCAAGCCCTTGATGCCCGTCTACTGGGAGAAGCGCTGGGAGCAGA ACATCCACGAGCTGCGCTCCGAATTGGGCATTACTGTGCTGAATAAAGCTTGATAAGGCTGTTTATTTTTAAGCCCATTT TTAAATTTTGAACTTATCGACTATTAGTATCGAGCAGTCCTATCGCGATCGTAGAATTGTGATCTGGCAACGTCGTGTAA CGTCGAACTGATTGTAGATGTTTCCACTTACAAAGAAAATTATTATTTGATTATAGCAATAAATATAAAAGTTGATGCAG TTTGCCGAAATAATTTAAAGATCGAGAAAAAAAACTTTATATTATTTTTTATATTAGAATTTGAGCACAGTGAGAACAGT CAATTTCCGCGAATGATCGCATCAAAACTTAACCCGATGTGGCAACTCCACTCGTTCTGTTTGCATTTGCTGATGTGTTG TTTTGTTTTTTTCGGTCGATCGTCTTAATGGCTAATTATCCCTAAGTTAAACACCATGACTGAGAAATTCAATGGTTTTA GCCACGACGAGATCCTCAAGATAACGGGCGTGAAGGAGGGCGGTGTGGGCAAAAGGCCTGCGAGTCTGGAAGCAGGTTAG ATCACTAAGCATGAACCCCAAAGCGTGTAAACAATTATCTGTTTATAATAAAAACCTTTGCCTATGTGTGCGTGAGCAGC GAAGCCGGCGCTCCGAATTCAACCCGGCATTCGGCGTATGCCCGATAAGATCTTCCGGCAAGCGGATCAGTTACGGAAGC AGCAGCAGCAGCAACCACAGCAACCCATACAAAAACCGGATGTGGCGAAGAAGACCAAATCGGGAACAGCGACTCCCACG GAAAAGCCACCGACTCCCACTCCCGCGGCGGAGGATGACGTAGCGAACGGATCGCTAAATTTGGACCGCCCACTCTCCGA TTCATTAATAGAGGCACTCTACCATGGAAATGCTACAGCTAGAGATAAGAAAACTCCCGCCACGTATGCAGATGCTGAAA CAACGTCCACCGACGACAGTTCGATTCTGAAGATCAGCGGCGGTGACAGCCAGACAACTAGCTCCACCGATGATGGCAGC ATCCTGCCGAGTACCCAGGACACCTCGCCGCGGGAGCGCCTCAACACAGACTCACCTTTCAAGGGCATCTCGCTGAAGGA CTTCGAACAGCATCGCCGCATGATCGAGGAGCAGAACAAGCAGAAGAAGCAAATGCTGTACCAGGCCATCGAGCAGCACA CCCAGAAGACGGCGGCGGAGTCGCGAAAGATCGAGGAGATCCGCCACGAGCTGTCCAAGCTGGAAAGTGATCTGGCCGTG GACGTAGCTCTGCTGAGGAAGCAGATCGACAATGCCTGCATACATTTTGCCAATGTAGAAAAACAATACGTCAAAATCGA GGCTCAGTTCTTGCGGGCCAAGATAGAGCTGCACAATGCATCCGAAAAGAAGGAGCTGCTCACAGAGCATCTATGCACAG TAATTGCCCACAACGAGGACCGTAAAGCGCAAAAGCTTACTGAATTGATGCAGAAAGTGGGTCTGTCGCCAACCGATGAT TGCCAACCGATTGATTTAACTCAACAAAGCCCCTAAACACGTAACACGTGTAAATTCAACTTCAAATCATCTTTAATTCG AATTTTCAGCGGTGTTTGCTTAACTTGACCAAAAGTATCAATTTGTTGCTCCCTGAAATATTATATTTTTTAAATTGGAA AATTATGTACTAATTTTCCATCGGCTATATTGTATTGGAATTGTAAACATAAAACACGCCTGAAATATGTCGAATGCGCT CTGATATTAGCATGTAATTAATGTTCATAGCCTAAGTGATGAAGTGCAAATTGCAAATAAAATATGTATATGTTATGTGT AAAGAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
