GTCGTCGAGGACTTTCCACCGCCGGAAAACTGTGAAAAACAGCGTGCGTGCGTGAGTGTGCGTGTGCTTCGAGGGAAAAA GCGAAATTCTCCGTCCAAACAACAGCAAACAAAACAAGCGAAGCTTCGTGGTTCCTGCGAGTTTCGCGTACCGCAAAACC GAATCATTAATAAAGAAAAGCCCTGCCGTCGACAAAATTGTTTCGCGTTGCCCACGGCTTGTTGACACGGCTTAAATGGG GCATTAAGTGCTCGGAGTTCAGTGCTGATATATGCAATTTCCGCTAATCACAAGTGCAATAATACCACACAATTCCCTGT GTGATGCCAACTGGCTAGCAGAACTAAAAGGATTAAGCTCCGGATTCACATCAGCCCAGCATTCCGAGTCGGTCGACAGG CGTTAAGCTGATTGCCCGGTGATATAAGAACTGTGGATAAACGAGGAAGAAATCGGAGGTGGAAGGCCTCTGGAGCAAGG AACATGGAGTGACTAGCAGCAGAAAAGTGGACAAAGTGACAGGCGATAGCCACAAAACAACACAGCCGAAAGAGGAAAGC AGGGCCAGTTGACAGGAGCTCAGCCAATAAAAGCCAACCAAACTCAGTCTGGGCCAAATTCGGACACACTGATGCAAAAA GGCGGCGGAGAAAGCCACCAGCAACAGCAACCGCAGCAAAACAAAACTGAAAACGGAAAAGTTGTTCTGAAACAGACTGC AGGAGGAGTTGCAGGAGGGGAATGAGGGGAGCAGCCAGCGGAAGCCGAGGCTGAAAAGTTCCCAAATTTGGCAGACAGAC CCCTTTCTGACCATCTCGTTCCCACTTTCAGCCGGAACAGTTAGCAGCAGCTGGAATTTGATTTTAACGCTTATTCGAGA GCTTGGGATCTGTTCTGGTTCTGAGTCTCTGTTTGTGGCACCAGCCACTGACAACCATCACCAAGGCAGGAGCAACTGGC AGGCGAATTAAACTCGGCACCAGGAAACTTCACCCCGGAAACCCCGCACCAACTCCCCCTGAAAGCTACGCATCAATGGC AACACATTGAGATTGGCTGTCAAAGGGGAGCAACCGGACGAAGTGGAGCTGTGGAAATGTTCGCCACTTGCGGCGACACA TGCTCTGACAATTTATTGACTCCTCGCGGCACCCACTAAACAAACAAATCCCGCTCCATCATGGAACTCCTTCCTCTGAA GTCGCCCCTCCCGATCCTGCTCCTATGCCTAACCATTTCCACTTCGGTCAGCGGCTATCTGAACATTTTCATCAGCCACC ACGAGGTGATGAAACTGATGGGACTCGAGGCGGACCTGTTCTATGTGCACGAGGGAGCCATCAACACATACGCAATGCAC TTCACCGTCCCAGTGCCCGCGGATGTCCATGAGCTGGAGTTTTCCTGGCAGAGTCTGATTGCCTACCCACTGCCATACGC CATTAGTATTGAGTATAACAACGACCAGGAGGCACTGGGCACGCCCACGTTGAGCATTCCGCACAAGGGTCTGGTGCCAC AGGAGATCGAGTCCTTCCTGGTTTACCTGCCCTGCACCGGAAATGCGAGCCTACAGATGCCCGTCAATGTCAACATGGTA GTACGGGCTCCTCCTCGATTCAACGACACCCGGCTCCACTTTAAGCGCAATAAGATCTGCGCCAAAGGCATCTCACCTGA GCCCAATCAATCGCCGGCGCCAGCCCACGCACCCTCCCAAGGACCCGCCCTCCTCAGTGCCGCCGCCTGTGCTCTGGGTT TGGTTTTGGCCGTGGGACTAGTGGCCAGCATGATGTATGTGAGGGCGCGCAAACAGCTTCGCCAGGACTCGCTACACACC AGTTTCACCACCGCAGCTTATGGCAGCCACCAAAACGTGTTCATCCGACTGGATCCCCTGGGAAGACCGCCGAGTGCCAC GGGATCCTATGCGACCATTGCCAGCCTCAACAAATACCCTGCGGACAGCAAAAAGTCGTGCAGCATATTCGACCGTTTCC GGAGCTCACCCACCCCCACGCCGTATGCCACCGCCCTGCTGCCCATGAACCTTGAACAGACCGCAGAGACGATTTACTCG AAGCCAGAGTCGATCTGTCCCTCGAGGATTTCCTACTACGCCTCCTCGCAACTGACCCAGCCTTGCAGCCTATCGACGCC GAAGAGCATCCGAAGCAACGGCAATGGCAACTGCACCAGCGGAAGTGGCAGCCTAAGCCTTTTTGGAGGCATCCCCACCG GCAGCACCATCACCATGGCCAGTCACGGGGAGAAGGGTAACCAACGGCTGCGACGGATAACCAGCGTCCAGCCCGGTGCC CTCAGCTACGAGGAGCTGGTCAAGGAGGGCACCTTCGGCCGGATTTACGCCGGCAAGCTGGGCGAGTCATGCGAGGCGCT CGTAAAGACCGTCATCGATGGCGCATCGCTCACCCAGGTGGCCTGCCTGCTGCAGGACGCCTCCCTGCTGATCGGCGTCA GTCATCAGCACATCCTGGCACCGCTGTTGGCCAACACGGAGCTGCCGGGTCCACCGGAGATTGCCTATCCCCATCCGTCC AAGGGCAATCTCAAGATGTACTTGCAAAAGTCGCGGGAATCGAGCACAGCATTGAGTACGCGCCAACTGGTTGAGTTTGG GCTGCACATCACCAAGGGATTGGCCTATCTGCACTCACTGGGAATCGTTCACAAGGACATTGCTACACGCAATTGCTACC TTGACGAAGAGTCCTATGTGAAGATCTGCGATAGCGCCCTCTCTCGCGATCTCTTCCCAGACGACTACGACTGTCTGGGC GACAATGAGAATCGCCCGCTGAAGTGGCTGTCGCTGGAATCCCTCCAGAAGCGGGTCTATGCCACCCAGGGCGACGTGTG GGCCTTGGGCGTGACCTACTGGGAGCTGGTCACTCTGGCGCAGATGCCGCACGAGGAGGTGGATATATTCGAGCTTACCA ATTACTTGGCTGCCGGCTTCCGGCTGGAGCAGCCAGTCAACTGCCCAGATGAATTCTTTACGGTTATGAACTGCTGCTGG CACTGCGAGGCCAAACAGCGACCTACGCCCTCACAGTTGCTATCGTATCTGCAGGACTTCCATGCGGATCTGGGCATGTA CATCTAAGCGAGACGAAGGACTGACGACGGATGAGCGGTATCCAGGTGGAGTCAAGCGGAGCGAAGCCCAAAATAAATTA ATTGAAACTTTTGCATTTTAAATGTGAGATTAAATGTGAATTTACCTGTTAGAGTTTGGCGCAAAACCAATTACACTGAA AGCCAGCTAACTTCAGATACGTAAGCACACGCATATACACACACCAGTACACTGGGATGGACCTCAATGAGTTGAAAATA ACTATTAAAATTAATAAAAGCAGTTAAATTAACATGTAGCTTAATATCACGGAAACTGCCTCAAGAAATATACCACAAGG CTATGCTAATAGGGTAAATTAACATGGGCCACACTGCGTATGAGCGATGTGTATACCAGCTAGTGCGCACAAATGAACAT CGAATGCTTTAAGGCCACACCGCGTATGAGTGATGCAATTTTCTGGCCTTAATGTTGAATCTTTCCTCGAACAAATGGCC ACACTGCGTATGCGTAATGCTTTAGACCAACGCACTGCCCTTGAAAGCCATACATCAAAGCAAGTCAAAAGTCAAATAAC AACTGAGGTCCACCCAACAATAGAAAAGCTATGATTTTCCAACCCGTTGACACAAGGCCCGGGTCGAACTGGCCACCCGA GTGGCATGTGGGTTAAGCGTTAGATAAAACTGACATTTAAGTAGTTAGCAATTGAATAACAAACACTCACACGCGGCGAA ACACATAGAATCACACAGACACATACACGAACCTTAAATGATAAATGAATGTGAATTATTGATGTGCATATTTTATTGAA TGTGTGTAGAGTGTAAGCACAGGACGAAGGATTGAACGGGAAGAGATGCGACAACAGGATGCGGAATCTTTTGTTCACAA ATGTTGTCCTCGAGATTGTTACGAATGCGATATATTTATTTACCTTGATTATTATTATTACTATTGCGTATGTCATAAGG GAGGAAGTTCAAAACAGAATCTGAATCAGGTGAAAAGCAAAAAAGAGCAAATGTTAAGCCGATGGCAAACAACCGATGAA CATTATTCACACCCACGACCACAAACACAACTACACACTCGCTTGCATAGAGCTCCCTTAGCACCTTAACCCCAGAATGC CCTGCATCGTTTACAACCTCCACAACTTGTCCCAACTTGCTCGTATAACGCACTAATTCAGTTTCGGAGAAACTTGTCGC AATCGTTTCGTTGCTCAATTATTTCGAAGTCAAAGGACAGGGGGAATAATCTCCGCTTCTGGCAGGATACGCCATGAAAA ATGCAATATGCAGCTGGCTTTTCGCTCAGTGCATTTGTGAGTGTCTGCGTTCGCTTCTCAAACTGTCACTTGGCCCCAAT CCGAGCCAAAATCTCCAGCTTAAGACGTCCCTGCTGCTTGTGTACGCCTTTTAGTTGAATTAGCCAGAAATTGTTTCTGT TTCTGTTTCTATCTCTGTATCCATTTCCTCAGAGTTGTGTATTAATTTGCCTTTCCGTTAGCCAACTCAGGCGGCAACTT AAAGTTGTCAAGCCCCCCCACAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
