CCACCGATCGCGACTTTGTGTGTGTTAAAATGACGCGCATATCGTTTTGGGCTAATAATATTGTTCACGTTTTATAAAAC AGATACACGGTTAAGCTCTGACCGGTCGTATTATTATCTGTGTGTTCTTGGTTGACACGTTTATTTCATTTATTCTCATT TAAAGCAACGATGTGAAGAACAGTGCGGGCGTTTCTCAACATAAGAATACATACAAACTTATGCGTGTTGGTGAGCAAAT AAAATAGTTCAAAAAATTACTCGGCCACCGCCTTAACCGTACAAACGGAAATACATAAAGTTTAGAATAAATACGGCCAC AATTAGTATACATATTTATTCATCGAAACCAACCCACCACAAGAGCATAACCAATTATGTTAAAGTTTATCAGAGGAAAA GGGCAGCAGCCCAGTGCTGACAGACACCGCCTACAGAAGGACCTTTTTGCTTATCGTAAGACGGCACAGCATGGCTTTCC TCATAAGCCTTCGGCTCTTGCGTATGATCCAGTTTTGAAACTTATGGCAATAGGGACGCAAACAGGGGCTTTAAAAGTTT TCGGTCAACCCGGAGTTGAATTGTACGGTCAGCATACTTTGTTAAACAATTCAGCATCGGAGCTTAATGTACAATTACTT GAATGGGTGTATGGAACTGGTCGCATACTTTCGTTGACGGCAGCGAATCAATTAATTCTATGGGAGCCAGTTGGAGCAAC GTTGCTGCCAATCAAAACACTACCGTTTGACGGCAAACTTAAAAAAGTTTCATCGCTGTGCTGTTCTCTCAGTAAGGATC TGCTATGGATTGGAACAGAAGGTGGAAACATCTATCAACTGGATTTACATACATTTACCATTAAGGAGCCTGTAATTTAC CATGACGTTGTGCTAGAGCAGGTGCCACCAGCCTACAAGCTAAATCCTGGTGCAATTGAGTCAATCCGCCAACTTCCAAA CTCCCCTAGCAAACTTCTAGTTGCATACAATCGCGGCCTTTGTGTTTTGTGGGATTTTGAAAGCGCATCTGTCCAGCGAG CATACATAGCCCCTGGACATGGACAGAGCGTTGGTCTTACAGTGAACTTCGAAGGATCTGAATTTACCTGGTACCACGCT GATGGTTCATACGCCACTTGGAGCATAGATAACCCAGAACCGCCGTCGAATGTTAATTATGTGCCTTATGGACCTGATCC ATGCAAAAGCATAAATCGACTGTACAAAGGCAAGCGAAGATCCAACGATGTAATTGTTTTTTCCGGCGGCATGCCACGGT CAGCATATGGTGATCACAATTGTGTGTCCGTTCACGCCAGCGATGGACACAAAGTGTGTCTTGACTTTACGTCTAAAGTG ATTGACTTTTTTGTGACCTTTGAAAATAATAGAGATGTCGCTGAAGTTCTTGTTGTACTACTTGAAGAGGAACTCTGCGC TTACGATCTTACTGACCCTAATATTTGTGCTATCAAAGCGCCATATCTTCACTCTGTCCATGCATCAGCTGTCACTTGCA ATTACCTTGCTTCTGAAGTCGTACAGTCGGTATATGAAAGTATTTTAAGAGCTGGAGATGAACAAGACATTGACTATAGC AATATTAGCTGGCCTATCACTGGCGGTACTCTCCCGGATAACTTAGAAGAATCTGTAGAAGAGGACGCGACTAAGCTTTA TGAGATTTTGTTAACTGGTCACGAAGATGGTTCTGTTAAATTTTGGGACTGCACTGGAGTGTTGCTTAAACCAATTTATA ATTTTAAAACTAGCAGCATTTTTGGAAGTGAGTCAGACTTCCGAGATGACGCAGCTGCAGATATGAGTGCGGAACAAGTC GATGAAGGAGAACCGCCATTTCGGAAATCAGGACTTTTTGATCCTTATTCAGATGACCCTCGTTTAGCAGTGAAGAAAAT AGCATTCTGCCCAAAAACCGGACAACTTATTGTTGGTGGCACAGCGGGCCAAATAGTTATAGCCGACTTCATAGACTTAC CCGAAAAAGTGTCTTTAAAATACATTTCAATGAATTTGGTCAGCGATCGTGATGGATTTGTGTGGAAGGGTCACGATCAG TTAAACGTGCGATCGAACTTATTAGACGGAGAAGCAATTCCTACGACGGAACGTGGTGTAAATATATCGGGAGTACTGCA AGTTTTGCCGCCAGCCAGCATAACATGCATGGCACTCGAAGCAAGCTGGGGCCTAGTATCTGGTGGGACTGCGCACGGCT TAGTTCTCTTTGACTTCAAAAACTTTGTTCCAGTATTTCATCGCTGCACTTTAAACCCAAATGATCTTACTGGAGCAGGA GAGCAGCTGTCTCGTCGAAAGTCTTTTAAGAAATCATTGAGGGAGTCATTTAGAAAGCTTCGCAAGGGTCGATCGACCAG GACCAACCAGAGCAATCAAGTACCAACAACGCTGGAAGCAAGACCCGTCGAGAGGCAAATAGAGGCTCGTTGTGCAGATG ACGGGCTAGGATCCATGGTGCGATGTTTACTATTTGCCAAAACTTATGTTACTAATGTCAACATAACGTCGCCAACTTTG TGGTCAGCAACAAATGCCAGTACAGTCTCGGTTTTCCTTCTGCATTTGCCACCAGCGCAGACCGCGGCAACTGCCGTCCC GTCGGCAAGTGGCAATGCACCACCACACATGCCCCGCCGAATTTCTGCGCAGCTTGCTAAAGAAATACAATTAAAACATC GTGCTCCTGTGGTGGGTATTTCTATTTTTGATCAGGCGGGTAGCCCTGTCGATCAGCTGAACGCCGGTGAAAACGGGAGT CCACCGCATCGTGTACTTATTGCTTCCGAGGAACAGTTCAAGGTGTTTTCACTTCCGCAACTAAAGCCGATTAACAAATA TAAGCTTACCGCTAACGAAGGTGCTCGGATTCGCCGCATCCATTTTGGTTCGTTTAGTTGTCGCATATCCCCGGAAACAC TGCAGAGTATGCACGGTTGTAGCCCAACTAAGTCCACGCGTTCACATGGCGATGGAGAGGCGGATCCTAATATCAGTGGA AGCTTGGCTGTAAGTCGTGGAGATGTATATAACGAAACAGCATTGATATGTTTAACGAATATGGGCGATATCATGGTTTT ATCAGTACCTGAATTAAAAAGACAGCTGAATGCCGCAGCAGTGCGACGGGAAGACATTAATGGAGTTTCGTCACTTTGCT TTACAAACTCTGGAGAAGCACTGTATATGATGTCTTCTTCTGAACTGCAGCGTATTGCTTTAGCCACGTCCAGAGTCGTG CAACCCACTGGCGTTGTTCCAGTAGAACCATTAGAAAATGAAGAGTCTGTGTTGGAAGAAAATGATGCAGAGAATAATAA GGAAACCTACGCATGTGATGAAGTTGTGAATACATATGAAATTAAAAATCCATCAGGCATTTCAATATGCACAAGGCCTG CAGAGGAAAACGTTGGAAGAAATAGTGTTCAGCAAGTTAATGGAGTCAACATTTCAAATTCACCTAATCAAGCTAACGAG ACTATCAGCAGCTCTATTGGCGATATTACCGTTGACTCGGTGCGCGACCATTTAAATATGACGACCACCACTTTGTGTTC TATTAATACAGAGGAAACCATTGGTCGCCTATCTGTACTTAGCACGCAAACCAACAAAGCCAGTACTACCGTAAACATGA GTGAAATTCCAAATATTAATATTTCTAATTTAGAGGACTTGGAATCGAAAAGAAATACGACGGAAACGAGTACTAGTTCT GTTGTAATTAAATCTATAATTACAAACATTTCTCATGAAAAAACGAACGGAGACAACAAAATAGGAACGCCAAAAACAGC GCCTGAAGAAAGCCAATTTTAACATTGACAGAAGCCGTAACCTACTAATTATTTTATACCTTTACAGAAATACGACGCAA ACGAGTACTAGTCCTGATGTAATTTAATCTATAATTACAAACATGTCTCACTAAAAATCGAACGGGGACAGCAAAATAGG AACGCAAAAAAATTAAATTAAATTAAACGCGATTTACATACAAACAGAAATGACAGAATGATAATATAAAATATTCATTT TTTATTTGGCTTAAGCGATGTTGTTGTTCCAAAACCATATAATTATTTATGATTTTATGTAATGTTTTCATGTATTTTCG CGTAGTGACTTTATACCCTTTACCCGTATGATGAAACGAAACATGATATTTGTAGAAAGGTACAAAGGATAAAAGTTGAC ATTTAAGGCCGATTGTAAAATATTAAAACGCAACCTGGATAACATGCCGATTAATTACTTCTGTTCGTCCGTATAAACAC AAATACCGATAAATTGGTCCGTCCCCATTTAATAAATTGTATATTAAAATGGTCAAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
