FB2026_02 , released June 18, 2026
Clone: Dmel\MIP23654
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General Information
Symbol
Dmel\MIP23654
Species
D. melanogaster
Name
MIP23654
FlyBase ID
FBcl0740062
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG4884, Dmel\CG42487
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    MIP_cDNA
    • cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,688
      NCBI
      BT125076
      Full Length sequence dataSequence Downloader
      AAAGAAGCTAAGGGCAAAAAGAAATTGCTGCCCAAACTGCGCAATATTCTAGCCAATCCTTACAAACAGCACAGCCCTCT
TTTATCGGAGGAGGAAGTGCAGCAGTTCCGTCAGATCCTTCAGAATGCAATTAAAAGCAGCGACGGGCAGACCAAATCGT
TTGCCACTCGATACGGAATACATCTCGGCCTGGAGAGCTCCCTACGCGCCATAAACTCGCGGCGTTTCTCCTGCCTATTG
GTTTCCTTGAGCCTGCGACCAACTCACCTCGTCCGTCTAATGGCCACCAGTGCATCTGTCAAGATGCCCACGGCTCCCAT
TTACGCGCAGCCCAAACTGGAGGAGCTTACCCAGGAAATCTTTGGCGTTAGAGCGCTTTCCTTGGCCTTGCCATTAGATT
TGAAAGCAAAAGCGAGGACTTGGAACAGTGGATTACTGCCCGAAAGAGAACACCTACACCTGCTAAGAAGATAGCCCCGA
AAACGCACAAAAAACCCAAGAAGAAACCCGAAATAATCAAACCAGCCGAAGAGGAAAAAAAACCGCAGCTCCCAGTGGCT
GAAAAGAAGGATTGGGGTGATGATTTCATATCCTGCTTCTCGGACGAACCCTCAGTAAAGTTGGACCGCGTGGATGTCCA
GGTGGAGACCCAAAAACTGGGCAATGCCCTCAGTAATTTGGCAATGAAAGCCAAAAGTAAAAAGCCGGTAGTAGAAGTCA
AAAATAACCCTGTTAAAAAAGAGAAGTCCCCCAGCCCAGAGCCCATGGAGGTGGAGCCACATGATGATGATGATTTCCTG
CCCGCAGATCTGCACAACTATCGCCCCCTTACTGTCTACCAAGTGCGTCCTAATCCCGACAAGAAACCCAAAAAGAAACG
AAACAAAAAGGCAAACAAGTCCTAGAGATGGTTGGCAACGCCACCGCAACAGCTGTAGTTTTTAGGTTATGTTATTCAGT
TTGTTATCGTAATTGTTAAGGCGCCCACACAAAATGCTCCGGACTTTTCGTCAAATAGCCCGACTAGGAAGATCCCTAAG
GACGCCATTTCCATCTAAAAGTCTGCCTAAACTCTCAACCACCTTAGAGCTGCAGGCTCCTGTACACACCAGTGCTTCAG
CGTGGAAGTACGACAAGAAATCCTCACGAGCATCGGATGACTTGGACAGCGACGACGAGGATGATGAGGATTTCAAGGAC
GAAAGGGACTCCAAGGTGGTCAAGACCAAGGTGAACTCACTGCGCGCCGATCTCCTACTGAAAGCTGGCCTGGGCATGGC
GAGAAACAAAGTGGAACTGAATTTCTACGAGAGCAAGATACGCGTCAATGGAAAGAAGCTGCCCAAAAAGAGTGCCCAGC
TGGAAGTCGGCGATGAAATCGACGTAATCCGAGGCTTTAGTCAATCGAATCCTTCCCATCTGGTCGTGGCCCGCGTCTCA
ATCCTCTCCGTCAGCGAGCGCGAGGAAGGACTCAGCGTCCACCTGAGGCGCTACAAATCCCTGCTGGTCGAGAATTACCG
GGGACCCAACGCCTTCAAATCCTCAGAGCACGTTGCTCATTAAAACCAATTAGAAATAAAGAGACTATTAAGAGTAAATG
GGTAAAGTCGAGTGAGTTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGAAAAAAAAAAAA
AAAAAAAA
      Library Information (1)
      Library: MIP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      MIP
      MIP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)