FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\MIP20544
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General Information
Symbol
Dmel\MIP20544
Species
D. melanogaster
Name
MIP20544
FlyBase ID
FBcl0740500
Feature type
cDNA_clone
Computed gene(s)
Dmel\smid
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    MIP_cDNA
    • cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,359
      NCBI
      BT124778
      Full Length sequence dataSequence Downloader
      AAGATATCGAAGCCACAGCTTCGCTTCGTTAGCATTTTGCGAGCGGCAAAGTGATTTAATTGTCCTCGTGCGAATGTCTA
GACAGAAGTAATCCTCGAATAATATGAAGAAAGCCAAGCCGCTGCTGCACGACCATCTAATAACGATCCGAGTAAAGAAG
GTGTGTCCAAATCCGTTGTTTATATCCACAAACTAGCCAGCTGCCCGATAGCCGGTAAATTCCCATGAAAGCAGCACAAA
AAAAAAAAAAAAAAACACACAAAAACCCTTTGCAGGGCGGCGATTTTGGGCAAGGGAAAACAAGTATCTGGAGGAGCACA
TCGGGGAAACCTACCTGGATGTGAAACAGATGACCAGGGAGTTGATGCAAAAGTATCCGGAATATTCACGACGCAAATTC
GGACCATTCAGACAGCTGGTGCACCAAGCATTCTCGATCATTTCGGAAAGCTATAACTTGGACAAGGTAAGCAGTTCCGA
GGAAGATTGTGTTAGCGAGGACTCCGAGCCACCACCCACGAATAGTGTGATGAACAATATGATGAACAGCCTGTATAGTC
AACCAAGAAAGCCGCTGGCGCCCAAGCCAATCAGCGAGCCCATCGATATATCCAGTGGAGACGAGAACGAAGATGACTCC
AATACAAAAACCACCAACGGAGATGGGGTAGCTGCTGCTGCTGCTCCTCCTCCTCCTACTCCAGCAGTGCAGGGCAGTGC
TTTGAAACGCCTGATGGAGGAAGTGCCGGAGATCGCAGTGGCTGCAAAGAAAGTAGCTAAACCGAATACTATTCACGTTA
GCAGTTCGGAGGCCATTCAAAAGCTGCACCAAGTTGTGGGCAACAGAGCGAAAAATCTTTCAGAGGACGCCGTTCCGCGT
TCCAAAGATCACCGTAATGTGCCTGGCTTATATCAGCAGTTGCACCAAAACCAAAGTCGCGATCGCCTTCGGAAGTTCAA
AAGGGATCTGGAGGTGCAGCACCCAACGGAAAGTTTCCGGGACATTGGTGGCATGGATAGCACTCTTAAGGAGTTGTGTG
AAATGCTGATCCATATTAAGTCGCCGGAGTTCTACTTTCAATTGGGTCTATTGCCTTCGAGAGGTTTATTGCTTCATGGG
CCGCCAGGCTGTGGCAAAACCTTTCTTGCTCGTGCCATTAGCGGGCAACTAAAAATGCCTCTAATGGAAATTCCGGCCAC
AGAGCTAATTGGTGGCATCTCTGGTGAGTCGGAAGAACGGATTCGCGAGGTCTTTGACCAGGCCATTGGCTACTCGCCTT
GTGTGCTTTTCATCGATGAAATCGACGCCATCGGAGGAAATCGTCAGTGGGCGTCCAAGGATATGGAACGTCGCATTGTC
TCGCAGCTGATAAGCAGTCTGGACAACCTGAAGGCCAACGAGTTTGGCCAATCAGTAGTCGTCATAGCGGCCACCACGCG
TCCCGATGTCCTCGACCCTGGACTACGTCGCATCGGTCGCTTCGATCACGAAATTGCCATACACATCCCATCGCGGAAAG
AGCGGCGTGAAATCTTGCGCATCCAGTGCGAGGGCTTGTCCGTCGATCCCAAGCTAAATTATGATAAGATTGCAGAGCTA
ACACCGGGATATGTCGGTGCTGATCTAATGGCTCTCGTAAGTCGTGCAGCTTCTGTGGCCGTAAAGCGTAGGTCCATGAA
GAAGTTCCGTGAACTGCACGCTGCTAGTGAAAAGAATATGACAACGGTCACTTTGGATGACGACGAGCCCAGCGAGGACG
CAGGTGAAACACCTGTGCCTGATTCAAAAGGTGAAGAAACTGCAAAAGATGCAGAGGCAGAACAAAAGGTAGATGGTGAC
AAGGAAACATCTGCTAAGGATAAATCAGAAGGCGACTCGCCAAATATTGAAACCCCCAAGAAAGCGACCAACGGCAACTC
ATCGATAAAGTCGCCGCAGAAAACACCGAAGAAATCAGCGGAGAAACCAACTGACGCAGCGATGGATGTAGACAATGTTG
CTCCCGAGGAGCCGAAAAAAGCCGTTGAGCAGGAGGTGGATTCCTCCTCCTCCAACGACGAGTACTACGAACCCACACTG
GCCGAACTGACCAATTTTCTTGACAACCCCCCTGAAGAGTTCGCCGACCCAAACTTTTGCCTCACTCTCATTGATTTCGT
AGATGCCATTAAGGTGATGCAGCCTTCGGCCAAGCGCGAGGGCTTTATAACAGTACCAGATACCACTTGGGATGACATTG
GTGCCCTAGAGAAAATTCGCGAAGAGCTTAAGTTGGCTGTCTTGGCACCAGTTAAGTATCCAGAGATGCTGGAACGTCTT
GGGCTGACAGCTCCGTCAGGCGTTCTGCTATGCGGTCCGCCGGGTTGCGGCAAAACTCTTTTGGCCAAGGCTATTGCCAA
TGAGGCTGGCATCAATTTCATATCGGTGAAGGGCCCCGAACTTATGAACATGTATGTCGGCGAGAGCGAACGAGCTGTGC
GTGCCTGTTTCCAACGTGCCCGGAACTCTGCACCCTGCGTCATCTTCTTCGACGAGTTCGACTCCTTGTGCCCCAAGCGA
TCGGATGGTGGCGATGGTAATAATTCGGGCACTCGTATCGTTAACCAACTGCTGACCGAAATGGATGGCGTCGAGGAGCG
CAAGGGTGTATACATATTGGCGGCCACCAACAGACCGGATATCATTGATCCGGCCATCCTGCGACCGGGTCGCCTGGACA
CCATTTTGTACGTGGGTTTCCCCGAGCAGAGCGAACGTACCGAGATTTTGAAGGCCACCACAAAGAACGGAAAGCGTCCG
GTTCTGGCCGATGATGTAGATCTCGATGAAATTGCTGCCCAAACCGAGGGCTATACTGGCGCAGATTTGGCAGGATTGGT
CAAGCAGGCCTCCATGTTCTCCCTGCGACAATCTCTAAACAACGGCGACACAAATCTCGACGATTTGTGCGTACGCAGCC
AGCATTTCCAGGAAGCTTTGCAGCAACTTCGGCCCTCAGTTAACGAACAGGACCGCAAAATATACGATAAACTGCGCCTG
AAATATGCTGCACCAAGGGTACCCACTCTAAATGATAAGTAAACCGATCAACAATAATGAGTTTGTTGATTTAGCACCAT
CGCGAATCAATTTAATTGTAAGTTTCGAAAATTAACTTTTAATTTTCGATACAAAATGCGATATGTTACCCAAAAAAACA
AAACCATTTAATCTATCTTGTAAAATGCGTAGAAGAGATATGTATTTAGTTTTTGGATTGTTTTAATTTTTTGTAGTATC
TTACAAAATAAAAGTTAAACTATTTGGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: MIP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      MIP
      MIP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)