Comment: 0-22 hr AEL
AAAAATCGATCGCAGATTTTTCCGTATGCGAATGCGATAGATAAAGTGGCACAAAATCTTTAACCAACAACAACAAAAGC AACATGCTTAATTCTACAGTATGTGTGTTTCATTGCCAAAGCAACGTTTACGCTAACATACTCTCCCCAGAGAGCAGATA AATACAAAATACACATAATAAAAATCAAAACCAAACATCAAATAGCAAATTACAAACAAAATCTAACACCACAACAACAA CCACAAAATATCGAAAGTAAAGAAACGAGACGAAAGAGAGCTCTTCATTATTTGTGTATCCGTGTGTGTCGTCTCTCTCG CGTGTGTGTCCGTGCCTATACCGTTGTGTGTGTGTGTGAGAGAGTATTGCTGTGTGCGTGCAGCAGAATCAATAATAAAA AATAAAAATCCAAAATAAAAAGAGCAGAAAAAGCAGCAAGCAAATGTGCAAAATACTACAGAGTATCAAAATCAACAACA AATAACAACACATCGGATAAATAACAATAATAGCAATACACTATAAGCAAACAACGTTGAGAAAATGAGGTGCTGCTGCT GTGCGAAGAAAACAAGCCAAGAACTATGACGCATCAAAATCATCAGACAAACGGCGCAAGTTTGGAGGATTGCCACACAA ACTTTTATGCCTTGACTGATTTATGTGGAATAAAGTGGCGGAAGTTCGTTAACGGGGAGCGACCGAACGCATCGAGCGAT CCTTTGGCGGACCCGATCCTGCGCTCATATTCGCGATGCATCCAGGCGGACATGCTGTGCGTGTGGAGGAGGGTCCAATC CACGAAGACGGACCACGCCGACCCGAATGCCTTAACCTTCGAGATGACCACGTCGACCAAGGTCCACCCGCCCCTTTCGC TGGCCGCCGCCAAGGAGCTATGGATCTTCTGGTACGGCGAGGAGCCCGATCTCAGTGAACTGGTCGATGCCGAGCTGCTC CGAGTAGCGGCCAACCAAGCGCTTTGGAATGGCACCTGGAAAGGAGCTCTCACCTACGAGTGCCGGTCGCTGCTCTTTAA GGCGCTGCACAATCTCATGGAGAGATTCGTGCTTACCAAGGACATTGTGCGCTTTGGAAAATGGTTTGTGCAGCCCTGCA CCTCCAGCGATCGCCTCTTTGGACGCAGCTCCCAGCACTTGTCCTTCTCATTTACGTTCTTTGTGCACGGCGACACCGTT TGTGCCTCCATAGATTTGCGCGAGCATCCCGCCGTGCGTCCGCTGACCAAGGAGCACTTGACCGAAGCGGCGGCAGCCTT TGCAGCGGCCAGTTCTCCGCCAGGATCAAATGGATCTGCGGCGTCAGCAGGGGGAGCTGTGCCTAATCCTGGTCAGGATC CAAATGGAGCCAGCATGGATGGACTGGACGGCGGAGAGGGAGCGGCCAAGGCAGCGCCACCTCCGCACGCGCGAAAGGTT ATGCTGGCGCCCTTTGGAATTGCTGGCATACTTACCGGCAATAGCTATAAGGCCAGCGATCCCATAGCCGAGAAGATCCT TGAGGATTGGGCCTCGTTTTTTCCGCTGTGCAATAAGGATAACACGGATGTGCCACCCGTGGTGGAAGTGGTGTCGGGTG GTCATAAGATGTATCATCCCACGAACTACGTACTAGTCACAGACCTGGACGACATGGAGCACATGGAATTCGTCGAGATG CAGAAGATGCAAAGCTCAGTGGCCGGAGCGGCGGCCGAGTCAGCTGTTCTGGCCTCGCTTTCCTGCCCACCGGGAGCAGC ATCTGCTCCTTCATCGATGGGAGCAGCTCCATCTGCAACAGCGGTTCCCTTGGGTCCAGCTTCCCTCAATGCGCCAGCTT TAGCAGGAGCAGGTGTCGGAGCCACCGCATCTTCCGCCGCCAAAGAGATATCCCGCAAGGCAGCTCCACAAGCGGTTAGC GCCCTGGAACGTCTCGCCTTCCAGCCCTACTACGATCAACGGCCCACCTCGGGCTTCACCTTCAATACCAACAATACTCA CATACCCGCCTCGGCAGCCGTGGAAATGCCGGAACGAACCTGGCAGGATTGCGTGATGAACACGCTCCATGTGGATGCAG CGGCGGCGGCAGCAGCAGTTGCTTCGTCTACGCCCGCCTCGGGAACTGGGTCATTATCCGCGGATGGGGACGAGAATGAG CAGAATAAACCGCCACAGGACTCCAAGCAACTGGTGCAGCAGCAGATCCAGCAGCAACAGCAACGCCAAAAGCTCTGGAA CTTTGTGGATCCCATGCAAAAGGCACCCTGCATATGCACCAAACATCTGGGAAACACACCTCATGGAACACCACACGGCG GCGCTTCAACGTACTCCCGCAACTCCTTGGGCGGCGATTCCTCGATGCCGGTGGCCTCCGTGGAATCACCGGCAACGCCG GCTCCCTCTCCGCATCCGAATTCGGCGCACTCGCAACCGACATCCGTGCCGCCCGCTGAGCAACTGCTCAATATGAGTCC TCATGCGCCAACTTCCGTTTCCAATCTCCAACAGCCGCCGACGCCTATCGACCACCTGCTGGACAAGAATACACCGGCGC CAACGCCAACGGACCAGCACGACAGCAAGAGCATTACGGCCTCGCCCTATGTTCATCAGACGCCAAGCGTGGAGCCGCCC TCGTATACGGATCATGCGGCCGGCGGAGGGCCAGCTGGTGGGCAGGGCCTTGGTACGGGTCCAGGTAGTGTGCCCGCCCA GCAACCGGCTACGCCCACAGCAGCAACGTCAGCTGGTGGCGCCGGATCTGGCGGACCTTCAAATGCAATTGGAGCAAATG CGGTTGGTACTATAAGTGTCAAGAAGCTTGAGATGCAGCAGCAGACTCCATCGGCAGCTATGGCCATCAAGCAGGAGCCT GGTGCTCAAGGTCGGGGGGTTGGAGGTGTTACCTCCACCACGGAGGCCTTGAACAACTTTAAGCGACTGTACAACCCGCC AAAGCTGACGTTAAAGGATCCGGACAGCTTCTACGACGAGGAGTGGCTCAAGGAGGTCATCTATGACTTTCAGTACCAGG AATACTGGGACTATAGCACGGTGAAACGTCCGAAGATGGAAAAGCAACGGAGACCTAGGTATGCCAAGAACCTTTACGAA GGACAAAACCACGTGAAGCCCGTGATGCCTTCGCCAGGATCCGTTTATGGCTCACAGTTGCTTTCCTTGGATGAGTCAGC GAGCCAAGCAGGAGGGAGAGGCGGTGGAGGACAGGCGGCGGGCAGCTCCGGCGGTGGTCTAGTGGGCATTGCCAACAGTA CTGCCAGTGGCAGTGATGTAGAAGCTGATGGTAGCAGCTTCTTCCAGGGACTGAACATTAAGACAGAGCCTGGATTGCAT TCCCCTTCTTGCAAGGAAACGTCGAAATCCTCGGGCGGCAACAGCAGCGGTGGAGGAAGTGGTAGCGGTGGTAATCTCTT CACAGCTGAAGGCTTGAATCCCTCGCTCAACGATCTGGAGCAGTTGTTTGAGACAAGCTCGAACGACGAGTGCAGCAGTG TGCAAATCCACACGCCACCCGATTCTAACAACCCCTCGAATGGCGGCTGCAGTGCTGTGACCAATACGATCGAAGACCTC AAACGGAGCACAGCGGTGGCCAGTGCTGCCGTAGCAGCGGCAGCAGCAGCTGCGGCCTCGGGCGCGGGCAATATCCAGGC GGAGGATCTTACCAAGATGTTTCCCACACCACCGTCTCACGAGCAGCAGCACCCGAACTCGAGTCCCTGTCAAACGGACG TGGTTATGACGGATCTCAGTGTGGACACCACCACAACCATTATAACCAGTAGTATCACCACTACTTGCAATACCACTATC ACGAGTAGTATCAACACCACCACAACAGCCTGTAGCAACCCAAGCAACAGTATTATGCTGGCTGCCGCACAAGCTCCCGT CACCGTGGTGGCCATCCAGACTGTCTCCAAAATGGTAAAGCAGGAGTATAACCTGGAGCTGGGTAGTCCTATGGAAGAGC CCATTAATGACTGGGATTATGTGTACCGACCTCCGCAACAGGAGAAGTTTGTGGGCTCGACGCGATATGCTCCATTGACC AACCTGCCCAGTCAGACACAGCCGCCGCTGACTCTGCCTACGGGATGCTTTTATCAACCCACCTGGAGTAGTCACAAATC AAGAGCAGCAACTTTGGCTAAAGCAGCGGCAGCTCAACAGCAGCAGCATCAGAAACATCAGGCCCTTCAGCAGCGCATCC AATTGCATCAGCAGAAACTACAGCAGCTCCAGCTTCAGAATCAGCAGCAACAACAAGCGGCAGCCGCAGCAGCAGCGGCG GCTAGTGGAGGAGTTGGCCACCAGAAGCACCAGCATCAGCACCTTCATGATCTCCTGTCAGCGGCGCCGAGGACACCATT AACGCCCTCTACTGTTCCGCAACCGTTGAGCAGTGGTGGCAGTCAGTATTTGCTGAATCAGTTGAACTGCCCACAAGCGC CACCTGGTGCCTCCATGCAGCAGCTAATGCACCGAGCGGGGATGTCACCAATTTCACCGGGACCCGGAATGGGTCCGTAT GCCGCCAGAAGTAGTCCAATGTCGAGGGCAACCCCCACGCATCCGCCACCGCCATATCCGTATGACTTGGCTGTGGCCAG TCCAGCTACCTCCACATCATCGTACTTGAACCGGCCGCTTCACTCGCAAGAGCATCCGCACATGCATGGCTTGGGCGGCG GAGCAACGGGAGTTGTGGGTCACGGAACAGGAGGTGGCGGGCACATGGGTATGGTAGCGTACACTGGCGATGCGGGCATT GTCAGTGGTGGAACAGCGATGGCAGCCGGATCCTCGTCCTTGCTTCAGGAATTACCAGAAGTAAATTCTGTCCTAGTTAA CATCCTGCTGTACGATACCGCCTTGAATGTCTTCCGGGATCACAACTTTGATAGTAGCAGTGTGTGTGTGTGCAATGCGG ATACGCAAAAGATTGGCAATATTCGCGGAGCGGATTCCGGAGTATATGTACCCCTGCCCGGGGTTAGCTTCAATCCTTTC CCTTCCGGAGCTGGAGGCGCTTTGGCTGGACAGCGAATGCTCAATGGACCCAGTTCCGCCGGTTTTGGCGGCATGCGGAT GATCAGTGCCTTTGGGGGTTCGCCAGCCTCAGCCTCGATGCCCGGAGCTGGCTCCGGACATGGCCATGGTCCAAATGGTG GCTCCAACTCGTCGTCTTGCACGCCGCCCAGCAGTAATCCCCATATCACCGGGTATGTGGACGATGATCCCGTGGAGTGT ACGTGTGGTTTCAGTGCAGTGGTTAATCGAAGACTCTCTCATCGCGCTGGACTCTTTTACGAAGATGAGGTGGAGATCAC GGGCATCGCGGATGATCCGGGCAGGAATAAGCAGCCCACGTTGCTCAGTATCATCCAGAGTCTTAGCAGGAAAAACCAAA ACAAGCAGGGACCTGGAGAAACAAGCTCTGCATTGGATAAAATTGGAGCGGGAGGATTGCCTAATGGACAACTGGAGCAA TTGGGTCATGCTGTATTTGACCTGCTATTGGATCAGTGCTCTATCATCCAAACATCTAGCAGTTCGGTGCACAGAGCACT TCAGTCTCATCGGAGGCGAATGTCCCGCCAACGAAGGATCTTCGGTAACAATGGAGCTCCCACTGCCTCATTGGCATCAA TTGCAAATGTTCTAGAGTTTATGGATGCACACGACGTTATAAGTTTGGCCCTGGAGCAGTCGAGATTGGCATTCGAAAAC CAGCGGATGGACAACATGATGGACTTCCATGGGAATGGTAGCAGTAGCTCGCATCAGCAGCAGCAACTGACAGCTTTCCA TGCGCCACCACCTGCTTTGCGTCACAAGCTAGCAGGTATTGGTGCTGGGCGGTTGACGGTTCACAAGTGGCCATATCTTC CCGTCGGTTTCACCCGTAGCAATAAGGAGATTGTACGCACCATGAATGCTATACAGCCGATGCTGCAGAATGCATTCCAT TGCAAATCCAGGGGCGGCTCAGGTTCCAAGGATGCCAGTTCGTACAATACGGTGAGTGGACCGCTAACCTGGCGCCAATT CCATCGACTGGCAGGCCGTGCATCCGGACAATGTGAGCCGCAACCGATACCATCCGTGGTGGTGGGCTACGAGAAGGATT GGATCTCGGTGGCACCGCACTCCATCCACTATTGGGATAAGTTTCTCCTGGAACCGTACTCCTATGCCAGGGATGTTGTA TACGTGGTAGTGTGTCCGGACAACGAGCATGTGGTAAATTGTACTCGCAGCTATTTCCGGGAACTAAGCAGCACCTACGA GATGTGCAAGCTGGGCAAACACACACCCATCCGAGGATGGGATGGTTTCCTTCAAGTAGGCGCTGCTCGCAACAACGTGC CTGCGGATCGGGAAACAACTCCCTTGGATGATTGGCTGCGTACCCTGGAACATGCTGCTCTGGCGGAGCAAATCCGTCGG TATGCAGTTGCCTTTATTCACCAGTTGGCTCCATACCTAAGTCGAGTGCCAAATGATAAAACGCTGTTGAATCCACCAGA TGGCAGTGGCAACTCACACTCTAAAGGAGGAAGCTCTTGCTCCTCGAATAGCTCTTCAGTCAGCGGATTGCCAGGCGGGG ACTTACCCACGGATAATATAAAGCTGGAGCCAGGTACAGAACCACAAGTCCAACCGATGGAGACTAACGAAATAAAACAG GAACCCGGAGTGGGAAAAGGAGGCACTGCAGCAGGTGAAACCAAACCAACTCTGATCCTCGGAGATCCGTTGGGAATGGG CGAGACTTTGGAGGACATCAACCCGTCAGCCATTGTCCTTTATGTGGTCAATCCGTTCACTTTCGCCTCGGATAGCTGCG AGCTGGAGCGTCTAGCTCTGATTGCTCTACTTCGTTGCTATGCGGAACTGCTGAAAGCAGTCCCAGATTCAGTGCGATCC CAGATGAACATACAGATTATATCGCTAGAATCGGTAATGGAACTGGGTCCGTGCGGCAATCGAAAGCGTTTCTCGGACGA GATTAGGTGCCTGGCTCTAAACATATTTTCGCAGTGCCGGCGACATCTGGTGCATGCCCAATCCGTTAAAAGTCTTACTG GCTTTGGTACGGCGGCTAATATGGAGGCGTTTCTCAAGACAAAGGATGAACCCAATCGCCGGGCCTACAAGATGTACACG GCTCCCTTTGTTCTGGCACCAATGCACGAGAGGAATGACAAAACGGACTTCTCCAGATCAGCGGGAAGTATGCATGGCCA GAATGAACATCGTTATTCGGTTATGTATTGCAATTACTGCCTAAGTGAGGATCAGGCTTGGCTTTTGGCCACCGCCACTG ATGAAAGGGGCGAGATGCTGGAGAAGATCTGCATTAATATTGATGTGCCAAATCGTGCTCGGAGGAGAAAAGCCCCTGCT CGCTATGTGGCCCTAAAGAAACTGATGGACTTCATCATGGGAATCATCTCGCAGACATCGCAAATGTGGCGTTTGGTTAT CGGACGCATTGGAAGGATCGGACACAGTGAACTGAAGTCGTGGAGTTTTTTGCTCAGCAAGCAACAGCTGCAAAAGGCAT CCAAGCAATTTAAGGATATGTGTAAGCAATGTACATTGATGTATCCACCCACGATCCTGAGTGCTTGCCTTGTGACCTTG GAGCCGGATGCCAAGCTGCGCGTGATGCCCGACCAGTTTACGCCAGATGAGCGCTTCTCACAGATTTCCATGCAGAACCC GTTGGCCACTCCACAGGACGTGACCTGCACCCACATCCTGGTCTTTCCCACTAGCGCCGTCTGTGCGCCTTTTACGCGCC AGTTCCAAAACGAGCCGCAAGTAGATGACGACTTTCTAACATTCGAGGAAGAGGGCAACGAGGACTTCAGCGATGCGGAC ATTGGAGATCTCTTCTGGGACACTCATATGGACAGAGTATCCAATCATGGTAGTCCCGGTCGCATGGATGACAATCGGAG TTGGCAGAGCGCTGGCGGTAATAATTTCAAGTGCACGCCGCCCCAGGAAGTAGAGGAGGTTGGTTCACTCAACCAGCAGC CCATATCAGTGGGTTACATGGTGTCCACGGCGCCAACCGGTCGCATGCCTGCTTGGTTCTGGTCCGCCTGTCCGCATCTA GAAGACGTTTGTCCGGTGTTTCTGAAGACAGCCCTCCATCTCCATGTGCCCAGCATACAGTCTGCCGACGATATTCTCAA CTCGACCAATGCCCATCAGTCAGGCAACGATCATCCGCTTGACTCCAATCTCACAGCGGATGTGCTGCGCTTCGTCCTCG AGGGATACAATGCCCTTTCCTGGCTGGCGCTCGACTCCAACACACACGATCGTCTCTCCTGTCTGCCCATCAATGTCCAG ACGCTAATGGATCTGTACTATCTGACGGCTGCAATAGCCTAAGGTCCGGGATCTCAGATCCTTTGGATCATCTAAAAAAC TAAATCAATCAAACGCAGTCGCATGCTTCCGTTTGCAGAACTTCCACTAGCTAACTAGCTATATCACATCTTCAAAGAAC AACAGAATACAGAACCGAAAGGATCTGAAGCTGAATCAACACCAAGTAACCCTGAATAGAAGTTAACAGAATCGAAAAAC AACAACAGAACAACCAGAGCAATAGCAGCAGCAGTTAACGCAACGCACAGATTACTTAGCATAATCAACAGGAAAACTAA ACATAAACCGATGAACCGATGAAAGCGGCGGATAAAACATTATCGTCTACCGATAAGTCTTGAACAGCAACAGCATTACT ATTTATTAGTCGTCGAGAATTTGTAAAAGTGTCAAGTGCAAGAATTGTTTGTTTAATTTTATTTTTATATATATACATAG GCTGGCGTTCGATTAGTGTTTAATATTTATAAATATACAAAGGGGAGCGAATGAGATAGCAAAAAGCAAACAACAACAAA CAACCACACACATATAAGCATATAGTTCATATATATACAATAGCGGAGTGGAGTTAACGCGAGAAACATATTAGCATAAG TAAAAAAGTTTGTACATATTTACAACCAATTCGCCGAAAGCAGCAAACCAGCAGCAACAACAAAGCAAAACAAAAGTGTA ATTATACAATTAGAAATTAGTTTACAACAAATTGGAGGAGCAGAAGAGGAGACCCTATTTAAATCTAATAAATTAAATCA ATAAATTGTGTAAATTTTTGTGCAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
