AGCTCTCATGCGGCTTTCATCCGAACCGCACCGTATCAGTTCGCAACGGAATTTTGCCAAAAAGATACTTTTGAAACTAC GTAACGAACTTCAAAGCGACACGTGGGTGTGAAAAAAGGCTATAAAAAAAAAACAGCTTCAAATTGCAGTGTTCGTTAAA TTATAAGAAATCAGGCGAAATATCTAAATGATGGCGAAGTGAAATGAATTCCGAGGAATGTCCGCAATTGGTCAACCTGT GAGTCCAGCGAATCCCGTGTGAGTGCTGTGCGTGCCCAGACGATGGCGGTGGGCAGTTCAATTATGGAGACCGTCGCTAT TTTGGCCCTGCTGCTTGGCCTGTTCATTCGAAGTGGCTACTGCATAGACTGTTTCAAATGCGTGTCATACAATGGTGCTA ACAAGGCCTGCGACGATCCCTTTCACAACAACTACTCAACGGCCATTTTGGAGTCGCCCTGCATGGGCGGCCGGAAGGGG CGTGACGGCCTGTTTCCGGCCACCGCTTGCATCAAGATCGCCGGCTATTACGATGGAACTGGCGAGACGATAACGGTGAG AGGTTGCGCCCTGGACAGCGGCACCCTGACCACCGACACAGAGATTATAAGAATGTCGCATTGCGGAAAGTTCTACTACG ATGACAAGTACGTGCACGGGTGCTTGCAGAGCTGCAGCGATGCGGATGCCTGCAACTCGGCCAGGAGGCAGGGCGCAAGT CCCGCAGACCTGGAGTCACTGATGAGGCACCTGCTCAGTCTGGTCGTGTTCCTATTGGCTGCCAGCAGGTAGCATAGTGG ATATCTGTAGGTACCGCATCCGGAGCAGAAGAGGCAGGCGGAAATATGGCTGCAAGGGAAGTAAGGCAGCCGGGAAGGCA TTCAAGTCGTCTTGGATGCACGTGGAAATCCTTTTCTTTTCCTTCGAATCTCTCTGTGTCTAGGATCGCAATCGCACCCA AATGTAAACGCACTCGAGCTGCTGCAGCAAGGACACGCTCGGAAACACACTCTCTCTTACACACAATAACATTATGGAAT TCATCAAGCAATTATGTGATTTTTAAGCGAATACTTAACAACTATCAAACAATAATTGATGAGCGCGAAAGGAAACTATT TAGATGCTAAGCATTCGAGTGGCGAAAATCAAAGAAAAACACTGCAAACCAAATTCAAATTCTAATTGCAATTATTTTTA GCTCCTAAGAAGGAATCTAGACTTTAAACGCTGTCGCTTAGTGAAAGGCAGGAATGGTTTACGATTTATATACGATGTGT TTTACCTTGGCATAAGTAAGCATTTTTCACCTCTCTAGATGAGTATTGTACATATACAAAAACAATTTATCTATTTGTTA AACGAGTGCTGAATTTTGGGCAGATTATAAATAGTTGTGTAAATGGATTAGTGAGTAAAATTAGCAACGAAAACTGCGAG CTACAAAGAGGGAGGAAAATTACTTTTTTTTGGGAGATTGATGTAATAACGTCATCTTTATTTAAACAGTTTTGTTTTAC ATTATCAAACCACTATTAGATTACTTGGTTTACTCGGAACTGTGTGAACTATTTACTATCAAATATTACTTCTATTAAGT CCTAAATCAAGCCATAAATGCCGGTCATAAATGACACAATTCGTTGCTATATAGACTTCTGAATTTAGACAGCCAACTAC TAACACTTACCGCCTTACCAGTCTGGTGCGGTTGATTCACCGGAAGATTGGTTATCATGTCCGGATAACCTGATAGGGTA TCGGTTACCTAGGTGTTGCCTAGCCGCCGCCAAAAATAACTATCGCAATTGCTTAGTACATTCAAGCAGCTCAATCGAAA TGGACGGTAAGCACTGGCGCATTCCCCTTTTCCGTTCGGATCCTCCAATTTAGCGGGATAATTTCGCTGACTTGCAGGTC ACAGCTGGCTTCACTTCAGCAACGGAGCAATCCCCCAGGCAGCCGTTGTGGCTGGTCACGATTCCGATGGCGACACCATC TTCATTGGCCGCGCCTTCTACTGCAACGACATGCTGCCGGCCAAGATTATCCCCAACAAGGGCAAGGCCTACGTGGCGTA TGCCAACCAGGAGGTGGAGCTGGAGAACTACGAGGTACTCAGCGGCTTCAACTACGAGTGGCTGCCGGCCGAGAACGGAG AGGTGCCTCCCGGCGCCGTCAAGGTCGGCCAGAATGTCGACGGAGAGACCTTGTACGCCGGAAGGGGCTACCATGCCGGC AGTTTGACCGTGGGCAAGGTGCATCCGTCCCACGGCTGCCTGTACATTCCCTACGATTCCGAGGAGGTTAAGATATTCGC CTACGAGGTTCTGTCCCGTCGCTTGGAGGCGAGATAGGTGATCCATTTTCAGTTATTGCCTGTTAATCACAAATAAATTC GACACTTTTTGTAGACGCTCGTGTGTTGTTATCTCCATTTGGTGATCATAAAACTTCCTTATCGTGCAAAGATTAATTTC AAACATAACTAAAAATAATAGAGTTGATAGCTTGAGAAATACGTGGTTATTATAATGTATTGTAGATGGTATTGGTTTTT CAGACTTCAACTTGTGCTCGCAGTTTTCGTCCATACCCCTTTTATGCAGGAAAAAGTTATAAGTTAACAAAACCAAATAT GACCACAGTGAAGCCACTAATTAACGTCTAAGTTGAAAACTCTCTTAATTACGCGATGCCTAAATACTTGATTGTAAAAT CGAGCTAAATTTTCATATGGATATGGCGAACCAATAGGACCAAACTAAATAAAACGAATACTATAACGAATGGGTCGGAG TCCACGGCAATGACATTCGATATGCATCGAATTCTAGTTTAAATTGTTTATGGTTCGCATGTCGGTACTTCATTTTTCGG ACTAAGCGAGCTTGCAATGGTTTCCGCAAATAAAAATCAACAACAACTATGTATAAACAAGTGAAGAAGTTTTGATAGAA CGCAACGGTTTTTGATCGGTAGCAGACAGGCAACACATCAAAATCAGGCAATTCGCATTTTCTCTCTGCTGAAATCGCTT GCTTGCCACGCAACTAAACGCAAACTAATCGAAAGCAAACACAATACGCACTTATTGGCTATAACGGCTCCCCGGCTAAG GGCCATTGAATTCTTCAACTCTCTAACACACAATGAACGACCAAGCAAAGTTTGTAAAAATAACACAAGAGTACATTAAA CGTTTCATGGCAAGGCGAATGCCAAACATTTTTGAATGCAAATGTATCTAGAATTTAAGGAGATCTTTATACATATACAA TTGAGGCGGCAGAGCGATGGATTAACGATAATTAGATAAACTAGATAACGACGCTACTTGAACTAATCAAATTGAATTGA AAATCGAAATTGAATCTGTTTACCCTGCAATTGAATGTGTGCGTACAACTTTTTCTAGTGCAACATTTTGAAGCATTACG AACTAAACTATCAGCATTTCTGAATAAAAAACTGCATACATATATTGAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
