AGCGGTGCCGTCGCAGTGCTGAGCGCAGAGTGTCGCAGGTCCAAGGACTCAGTCATCGCGTAGCCGCCGTGTGTGACGGA CCGACGCCGCTTTGTGGCCGATTTAAAGCGAGTCTTGATGCGAGAAGTGCATTCAATTAACATAAAATGTAATTAGGGAC CCTCTCTCCGCCTCTCTTAATCAAACGCAAACATCGGAAACTGAGCCGCGGAGGCAAAACGAATGCCATTAAAGCGCCTG CGAATAAATCACGGCATGCCAGTGCAGTCGAAACTCAATCAAGTTGAACGGAAATCGTCGAAAATTAAACCAGGCGCAGC ACCGGCATTGTGAATGCAGCATACCACCGCCATTCTGTACATTCTAAAAATAACAAACAACTCGAGTGCGATAAAAACAG CCGTTCCTACCAAGGTATAAAGTGCTAGCGTAAACACAGCCGAAGAAGGAGCGCAAACAAACATCGGAGCCACCTAAAAC GGCGAACAAACAGCGGCGAAGGTTCCTGTACTGTGCGGAATCCTCGCTGTGTCGGTGTATTGGTGTACCGGTGTACCGGT TTGTCGGTATATCGCTCTGTGTGTGTGCCAGTGTGTGTGGGCCAAAGGAGCCAAGCAAAGAGTCAGAGCAAAAATGTGCA AGTAAGGTGCAGCAAGCAAAGTGCAATGCCATCCGTGAAATCCGCCGAAAGTGAGATTCTTCTTGGGATTATAAGGAGCG ACAGCATGACGAGCGGCAATTCCCTGAGGTCCTGCCTCCTGCTGGCCACCATCCTGGGATTACTCTGCCGGACGAAGGCT TTGCCCTTTGAGTATTTAGACGAGCACGAGGACTTCAACTACGACCTGGACACGGCGCAGTCCCAGGCCAAGTACGACGC CCGTCTGCTCTCGCAGCAGATGCTCAGCGATGCAGAGCTGCAGCGGCAGGGGCTGAGCGACGGCCAGGACAACGCCCTGG ATGGCGACTCCGCGGCAGCTCAGGGGACTGGAGCAGGGTCCCACTTGGATGCGGTATCCTCTGTCCACGACGACCTGGAG CCGCACAGCAGGGCAGCGGCGTGCTTCACCAACGGGCACAAGTACACGCACGGACAGAAGGTTCCGCGCCTGGATGCCTG CGAGGTGTGTCTCTGCATGGACGGCGAGATCTTCTGCTGGTGGGAGAAGTGCGATAAGGCCAATGTAAACAAGGCGAGGA CGGCGGGGGACAACGCAGGACTTGGACTTGGTGTCGAGGACGACGGCGACGGCAATGGCAATGGCGATGGCGATGGTGAC TATTCAGATCCATATCGCCACGAGAGCACAACGGGAAAGTCAACAAAAGTGCATAAAGCGGCGAGGAAAGTTGGCAAGCG GCATAAGCATCGCAAGAATCAAAAGAATTTTAATGACTACGAAGTTTACCACAGCCAGCGGGAGAAGCAGCAGCAGCAGC AGTCGGATTATAAAAAGTCCGCCATAAAGCAGCAGCTCCAGATGCAGCAAAAACACAAAAGCGACAAGAGCGGTGCTGGC AACTACAATATAATCAAGCAACACAAACACGAGCAGCAGCAGCAGCAGCTCAAAATACCGCAGCAGCTACATCAGCAACA GCAGCAACAGAATGTGGCAGCTTTGGGTGTTAATCACGCAGCAAAGGCAACGCATTATCAGCAGGCAGCTTCGACGCCCC TGCCAACTCCCCCACCCCCGTCGGAGCACCCGCAGCACAGCCACCACCCACACCAACAGGCGCACTCGTCCAGCAAAATC CTCAACTTCCCCGAGAACTTGCCAGCCCTGCTCTACTACGACTACAAGACGGAGGAGCACGAGCACCACCAGCACCAGCA CCACCAGCAGCACTTGCTGCACGAAAAACAGCGCTTGCTGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGATGG TGCAGCAAGAGGCGTTGGCGCGACAAAAGGCATCTGAATCCGCATCTGAGCCCGAATCGCGGGCCGGAGGCACAGCAGAG GGAGGCGTTGAACCCAGCGGAGACTTGGCCGCTGATAAAAACTCTGATGAGGCGGAAACCGACAGCGATATTCTGCCAGA GCCGCCTACAAAGCAGCCCAGGGCAGCTGCCACACAATGGCCAACTCCATCTAGTAACAGCTCGGCCAGGGCGTTGATGA CGAGCAACGTGGCATCCACATCCACGGCTGCGACAACGACAACGACAAAGACATCGAAAACGAAAACGACAACGGCGACA ACGGGAAGGACGACGACGACGACAACGGCGACAGGCACAAATGAAATGGTGACAAGCACGCTGTCTGGAATGGAGAAGTC CGGGGCCACAGTTGCAGCCACAGATCTTGGACAGATGCAACCCGATCGCCGTGGACCAGACGCGGAGCGAGACGACGCCT TCCACCGCTGGCTGACATCCACCGAGCTGAATGCTGACAACACAAACTCCATGGACGACAGCCTGGAGCGGGAAACGCCG GCATCGACAATAATCGATGATGTTGGCACGGCCAACAAGAGTGACAGGAGCATCGGCGGCATCGGTGGCATCGGCAAAGA CAATGGCAACGACGCCGTCTTCTTTCGCAGCTCGTACAACGATTACAGCAGCGAATTCAATGGGAGCGTTGTCAATATTG ACATTACACTAACTGCAGTTGATGTGCATCCCCGTCGCCAAACGGATTTAATTGCAAATGGCAACAGAACGTCAGGCGCT AACGACAATGGCAACAGCTGTAGCAGCCAGGTTGGAGCAGCAGGAACAACAATGAACCCAGTGGCAGTCAGCACCAGCAG CAGCACCAGGAGCAGCAACAATCAGGATCAGCCTCAGCAGAGCCCTGTTGTCCCGCCGTACACCCTGACAACGATTATAA CAACTGCGCCGATGGCACCAGGGCGTATGTGCAATGTTTTGGGCAAACTGTATAAAATTGGTGACATTCTGCCGCAGGAC ACGGGCAACTGCCTGCAGTGCATTTGCACGGATGCCGTGACTCCCGACGAGATGCCGAGCGTCACCTGCAGTCCGCACAA TTGCCCACCGCTGGTTCTTCCGGATCTGTTCGATGCGACTGGTTACTGAGGTTACGGGTTTGTGCTGTAAGTTGCTGCAA CGGCAGGTGGCAAGTGGCAGGTAGCAGGTGGAACGCCCCTGCGGCATGAGCTGTGGGAGTTTTTGAATAGATCGAAGCGT GATTTAAATTTTGAAACCGAAAGCCACAACTGCAGCCAGCAAACGAAATAGAATCGAAACGAACGAACAAACGAACGAAT CGAATCTAATCGAATCGATTCGAATCACACTCACAACAACTGCACTACAGAACTCACTCTTGAATTGCATGTCACGGTCT CAACTAACTCAACTCAATATGAATTGTATGGATTTCAGTGTAAAGCCCTCCCTATCACGAGTGCCCCAAGCACTCTCTAA AACGAAACCTATCAGCAGACATATATTATATTCGTACCAAACTATATAGTATCGATCCAAGCCGACCAGAGGGGAGAGAG GGGAATGAATAAAGGAGAAGAAGGAAATCGCAGTTGGCTCACAGTTTAGACTTTAAGCTGGATGTTTATGCATTTTATAA GAATCCGTATTCATCTCTCTCCGCAACAAAAATCCATTAATGTATACAGCAGTAGCAATCGTAAAGAATTTATAGATGTA CCAAACAGAAGTATATAGTATATACAAACATATACGAGTATCTACTCTATATCTAAATCTAATATCTAAACCTATACCTG TGCGTAGCGAGTTCGGTGGGATCAGTTTGATTACTTAGATTATTTCCCGACTGAGAGGAAACCGTGTAGAGTTTTCAGTG TATTTATCAACGGAGAGCCCCAAGTGAAACGAGTAACTTATACAAATATAGGAAACTAAATCTAAACGAAAAAAACTGAA CAATGACAAATAATGAACAACGTGTGTAACGTGTGTATTTTAATTTTGATATTAAAAGCAAACAACGTTCTCCTCATTTG CATGCATGTTATTGTTTCGCGCTTCTTTGGAAATTAAGCCATACGTAATCTATGAATCGAAAATTGACGTTAGGAGCAAT GTATGGACATTGTTTAAACAAACACAAAGTTGGCAAGGAAAGACACCATATCGAGTAGCTATAACATTTTAAGGAATAAT CCCCACTGTATTTAATATAAACTTAGAATTTCAAAAATTGCGGAGATTACCGAAAATCTATTTGTTTTGGTTTCTGGCGA CAAAGACAGCATTTATTTCAAAGCAAGTTTAGGCGTGATAAACATTGATTTATTCGAAAGGGGTTAAAGCGGAATATATA ATACTTGAAAAGTAGAAAACAATTTCGATTTTAAGGCAAATCAAAATACATTCGAGCGAGTTTTTTATAACCGAGCTCGA ACAAAACTATACTCGTATGGGCATGTGAACCATGGAATCAAAATGCAAAATATACGTACATGCGAATATACCGATGCAGA CAAACGTAAATATTTATTATTGATGTTGCACAAGAGAGCGTATGGTAAATTCGAAGAAAAATAGAAAAATTCATCTTGAA CGAGCATATTAAAAATGTATACAAATATGCGTATTTATGTATGTGTAAAATAAATGTAACTACAACTCTCTACTAATCTG CAACTCGCACACAGATACAGATGCACCGGCATCCCTGTGTTTAAATGGAATTTGTGTTTGAAATCAAATTGAAATCAAAT CGACATGAATAAACCGAAACCCAACTAAGGCATTTCAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
