GCCACGGAAACAAAGCGGGTCTTATAAACATCAATTTCGCACAGCAAATAACGTATATTAAAAGATATTAAAGTTTTTAA ATTAAAGTTGTATCAAGCAATTCATCTAAGAGTGTTTAAAATGCTTGAAACTTCCTTGATTTAACACCTTGATTAAGTCA AGTTACACTTAAATCTAAGCCAACAAAAAATCAATTAAAATTTGTGTAATACATTTAAAGTCGGAACCCCAGTGCTGACA GCATGGAAACGGAGTCTCTGAACCGCAAGAACTCCTCGCGGAAGAGTTCAATCGTGAATGGGAATGGAGATGCCTCTGCC AAGTTGACCAACGGCGATGGAGATGGAGGCGGAGACGGAGGCGGAGAGGTGACTCTGAAGGCCAAGATGAGCCTGCTCAA TGGATGCACCGTCATAGTGGGCTCCATCATCGGATCGGGCATCTTTGTATCGCCCACGGGAGTGCTGATGTACACGGGCA GTGTCAACCTGGCCCTCATCGTTTGGGTCATTTCCGGCCTTTTCTCGATGGTGGGTGCCTACTGCTATGCGGAGTTGGGA ACGATGATCACCAAGTCGGGTGCTGATTACGCATACATCATGGAGACTTTCGGACCCTTCATGGCCTTCATCCGACTCTG GATCGAGTGCATGATCGTGCGACCCTGCTCCCAGGCCATCGTAGCCCTCACCTTCAGTACCTACGTCCTGAAGCCCTTCT TCCCGGAGTGCACTCCTCCGGAGGACTCGGCCCGCCTGCTAGCCGTCTGTTGCATCCTTGTGCTAACCCTAATCAACTGC TGGGACGTAAAATGGGCGACCGCTGTGCAGGATATCTTTACATATGCGAAACTGCTGGCCCTGTTTATCATCATTGCCAC TGGCGTTTACCAACTTTACCTCGGCAACACGCAATACTTTACGTTTGAGAATACGGACACGAAAGTGACCTCCATCGCGC TGTCCTTCTACTCCGGTCTGTTTGCCTACAACGGATGGAATTACTTGAACTTCATCATTGAGGAGCTGAAGGATCCCGTC AAGAATCTGCCCCGCGCCATCGCCATCAGTTGCACCCTGGTGACCATTGTCTATGTCATGGCGAATGTATCCTTCTACAC CATCCTGTCGCCGGATGAGGTCATGGGCTCCTCGGCCGTGGCGGTGACCTATGCGGAGCGGGCCTTCGGCATGCTGGCCT GGACCATTCCAGTTTTTGTGGCCTTGTCTACGTTTGGAGCAGTGAACGGCATTCTGCTGACCTCCTCCCGACTCTTCTAC GCCGGAGCCAACAACGGCCAGATGCCCGAGATCCTCACCATGATCCAGATCCAGAGATTCACACCCACTCCCGCGGTTTT GGCCATGGCGCTTCTATCGATGTTGTACCTCACCGTTTCGGACATCTTTGCTCTGATTAACTACGTCGGCTTTGCCACTT GGTTGAGTATCGGTGTGGCTGTATTGTGCCTGCCTTGGCTGAGATGGGCCCAACCCAATCTGCCACGTCCCATCCGAGTG CCCATGGTGTTCCCCATTGTCTACCTTATTGCCACCATATTCGTGACCGTGGTGCCCATGTACGCCAGTCCGGTGGAGAC TGGATACGGCATCCTTATGATCCTGTCCAGTATACCCGTCTACCTGGTGTTCATCGCCTGGAAGAACAAGCCCATTTGGT TCCAGAAGACTATGGGCGGACTGACACAAGTTCTGCAGAAACTGATGATGGTTGTGCGTCCAAAAGCGGCGTCAAAGTAA GCCTCACACGATTCCTACAGAAAATGCTCATGGTACTCGGAAAGCAGACGAAACCAGCTCAGGTGTAAAGGTAAAGAACG TTCTTCGGGGAAAATAGCGGAGATAATATTTCAAGTTAGCCAAGAGCAGAGAAAAGCATTTAAAAGCACACACTTTGATT GCAAGATGCATTATATAGACAACGTAAACGCTTTTGTGTAGCCAACCAATGATATACATACGAAATACACAACAACTTCT GCCAGCTCCTGGGCGACTATACGATATATACCATATATATATTTTTATATACACATGTAAGTCTTAAGTTGAAGCAAGGC CCAGGATTACGAGGAAGTTCCCAACAGAGGTCTGGATCCAAATTTAATATAAAGACTGTGGAAAACTACAAGGAATCAAG GAATCAAGGTGTCCAGAGCTGTCCGTAAATTGGGTTCGAATTTGGATTTGGCATTTTTTTAGCAGAGCTGTGCAAGCAGA ACGAGTCTTTTAGGATTTTCAAGTATTCTCGAAAGGTGGTTATGCAAACTGTGAAGCCCCGATAAAAGCTATTGTTTATT ATTAACTGCATTTGAAAACTAATTTTTTGAGAAATTTAGTAACCATGCTCTAGGCTTGGCTTGATCTAGAAAGCTAGAAG AACGCTTGAACATACTCATCGGAAGGATCTACAAAGCCCATCTTAGTACCCACAAATGAACATGAACATTTTAGAGCACG ATTGATTACTTTTTATTGATTTAAACGAATCTAGCTTAAGTGACACCGCAATTAAATTTCACTTGTCTAAGTTAATACAT ACATTTAGGTTGCTCAGTTTAGGTTTCACATGTGCATCGTAGAGGGTGCTGCCTGTACAAAGCCCAAGCCAATATACAAA ACCATCGCATAATGCACATATCCAACAAGAGAAACGATTAAAGTTGTTGAACGAGAACAGGAACATTGCCAACATACATA TTAATATACTGAAAATAACACTATATGCAAATTGTTGTCTAATTACGAATCCAATCGTATCGAATCGAATTGATCTAGCA TTGATTTAAACTTTCATTAGTTGTTGTTGCTTTGAGTGTTCGTCACTTAGTGTTATTCATATGAAACTATTATTGCCTAT TTAGCATTCAACTAATTAACTGTTACACATTAAATATGGAGCCGCAGCTGGGGAATCAGTTTAACTACTATGAAACATAT TGTTGACAATAATATTGTATTTATATATAAATGTATATTATATTATATGATGTATACCCTACAACCTCTGTATTTCTGGA AATAAGAACGATAACGAAATCTAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGTTTTTTTTTTTTTTTTTTAAATGCTTA TGAGTCGGCATTTTTAAAACTACACACAGAGTGAAACTTTATTCAACAACAGTAAACATATTTATGTGTATAATGGATGT ATTATATTAATAATTTTCCAATGTGGCTGGTAATTGAAGAGCGAAAACAGTAGCATTTCAGAGCATAGCGCATATGTATG TAGGTGATGCTCCACCGGGGAGCAATATATATATAAGTTATGTGATGCCATTTTCAGGCCGTTTCTGAAGTGTCTCACCC CATTCTACCGCGCCCTAATCTTTAACTTTTACGTAGAGTGTGGGTGGGTAGTGTTCTTGGTGTTCCACATTTAATCCTTG CTCTGCATGTACTTGATCAGGTCGATGACGCGGTTGGAGTAGCCAAACTCGTTGTCGTACCAAGAGATCAGCTTCACGAA CTTGTCGTTTAGCGAAATGCCAGCCTTGGCATCGAACACCGACGAGTGGGTGTCGCTGAGGAAATCGGTAGAAACGACCT CCTCATCGGTGTATCCCAGGATACCCTTCAGGGGTCCGTTGGCGGCCTCCTGAACCTTGGCCTTAATTTCATCATAGGAC GCACCCTTGCCCAAGCGCACGGTCAAATCGACCACGGAAACGTTGGGAGTGGGCACACGGAATGCCATTCCGGTGAGCTT ACCGTTGAGGGCGGGGATAACCTTGCCCACGGCCTTGGCAGCTCCAGTGGAAGCTGGAATGATGTTCTGGGCAGCGCCAC GTCCATCACGCCACAACTTGCCGGAAGATCCATCGACGGTCTTCTGGGTAGCGGTGGTGGCATGAACGGTGGTCATCAGA CCCTCGACGATCTCGAAGTTGTCGTTGATCACCTTGGCCAGAGGAGCCAAGCAGTTGGTGGTGCACGATGCGTTGGAGAC CACCTTCATGTCGGGCTTGTAGGCATCCAAGTTGACGCCGCAAACGAACATGGGAGCATCGGCGGATGGGGCCGAGATGA TAACCTTCTTGGCACCGCCCTTCAAGTGAGTGGATGCCTTGTCGATGGTGGTGAACACGCCAGTGGACTCCACGATGTAT TCGGCACCAGCGCTGGCCCAGTTGATGTTGGCCGGGTCGCGTTCGCTGAAGACGGTGATCTTCTGGCCGTTGACCACCAG GAAACCGCCCTCGGCGGCAACGGTGCCCTTAAAACGTCCGTGGGTCGAATCGAACTTGAACAGGTAGACCATGTAGTTCA CATCGATGAAGGGATCGTTGACGGCCACAACGTTGGCGCCCTTATCAATGGCGGCGCGGAGAACCAAGCGGCCGATGCGA CCAAATCCATTGATACCAATCTTCGACATGGTTAACTTTTTGTTTATCTATCGGCTTGCTTGCAAGGAATCGAACTTCGA GTTCTACGCCAATTTCAGCACCGATTGCACCGACAGCAACAGCAACAACATGAGCCGAAAGTTCTGCGTGGGAGGCAACT GGAAGATGAACGGCGACCAGAAGTCCATCGCCGAGATCGCCAAGACCCTGAGCTCGGCCGCCCTCGACCCCAACACGGAG GTGGTCATCGGCTGCCCGGCCATCTACCTGATGTACGCCCGCAACCTGCTGCCCTGCGAGCTGGGTCTGGCCGGCCAGAA TGCCTACAAGGTGGCCAAGGGCGCATTCACCGGCGAGATCTCCCCTGCGATGCTGAAGGACATCGGCGCCGACTGGGTGA TCCTGGGACACTCGGAGCGTCGCGCCATCTTCGGCGAATCGGACGCCCTGATCGCCGAGAAGGCCGAGCACGCCCTGGCC GAGGGCCTCAAGGTCATCGCCTGCATTGGTGAGACCCTGGAGGAGCGCGAGGCCGGCAAGACCAACGAGGTGGTGGCCCG CCAAATGTGCGCCTACGCCCAGAAGATCAAGGACTGGAAGAACGTGGTGGTGGCCTACGAGCCCGTCTGGGCCATTGGCA CCGGCCAGACCGCCACACCCGATCAGGCTCAAGAGGTCCACGCCTTCCTGCGCCAGTGGCTGAGCGACAACATCTCCAAG GAGGTGTCCGCCAGCCTGCGCATCCAGTACGGTGGATCCGTGACCGCCGCCAACGCCAAGGAGCTGGCCAAGAAGCCCGA CATAGATGGCTTCCTGGTCGGAGGCGCCTCCCTGAAGCCCGAGTTCGTGGACATCATCAACGCCCGGCAGTAAGCAACGC GATCTCAAGCATCAGATCGCTTTCGGCCAGCGAACTGCCCGCGAAGGAGGCACTGGAGGGCAGGACTCCATACTCCTGCG CGCGAATGCATTACGCACACACCTAGTTGTTGTCACCACCAAGTGCAGTTCCAAATTCATAGTTACCTTGCGAGAATGCT ACTCACATCCTTGTTGTTGAACGTATAGTAGTCCATTGTGTAAATCTTAAATTATTCAGGCAATAATTATGCGCTACTCA TTTACTTAAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
