FB2026_03 , released September 17, 2026
Clone: Dmel\DPiM_LD22858
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General Information
Symbol
Dmel\DPiM_LD22858
Species
D. melanogaster
Name
DPiM_LD22858
FlyBase ID
FBcl0742217
Feature type
cDNA_clone
Computed gene(s)
Dmel\Bub1
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    DPiM_cDNA_bait_cDNA
    • Cell line expression plasmids encoding full-length open reading frames with C-terminal FLAG-HA tags under control of the metallothionein promoter.
    Strain
      Vector
      Tissue Source
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,785
      NCBI
      AY069508
      Full Length sequence dataSequence Downloader
      AAAAGCAAGAAAAAATACAAAATATCTTTGAGCATTGCAAACTGTTGAAAAATCCAACTGAAAATCATGTGTATGTGCCA
CCAGTGACAGCCGAAGACGCCGAGGGATTAACTGAGTTAGTGGACCCTAACCAGTCCAATCGGCAAGGGGCACTACATTC
CGCAATCCGAGACTTTTTGGTGGTGACCCGTAGAAACCACAGTCTCGCATTCATTTGTTTGCATTACGGCGCAGCGGGAT
CAGAGGATTCCGCGTCCGGCCGAGATGGCCATGCACTCGTACATGCGCCAGGGATCGGGTTCCGGAGGAGGAGCAGGAGC
AGTCGCAGCTGGAGCACCGCCGCTGGCCAGCCCGGACGAGATGCACGGCTTCCTGAACGACAAGCAGGCTTGGGAGCACG
CCATATCCCTGTACCAGGGTCCTGATCCACTCGATCATTGGTACAACTACATCTGCTGGTACGAGAATCACGCGCAAAGC
GATCCGGAGCTAAAGTACCGCGAAACTCTGGAGCGCTGCCTGACGGTGTATGAGCACAATGACTACTATCGCCAGGATGT
CCGTTTGGTGCGGCTGTGGCTGAAGTACATAGCCATGCAAACGGATCCACTCCATTTCTACCAGGTGCTTTTTCAGCGAG
GAACTGGTCGCCAGGTGGCCGCCTTCTACATTGGATGGGCTGCCTATTACGAGTCTCGCGAGGAGTATAAGGATGCGGAG
GCGGTGTTTAACTTGGCCTTCCAGGAGAAGGCGCAGAGCACTTCCGAGCTGCAGCACGCCCACACCAAGTTCGCCTATGC
CAGGTCTCTTTTTTACCAGCGGCAACAGCAGCAACAACAGCAACAACAGCAGCACCAGCAACATCCCCCGCAGGATGCAC
TGCAGCAACTCACGAACTATGCACAGCAGCAGATGCCACAGAGCTATAATCAACACCGTCCCCAGCCATATCAGCAGAAT
GTCTATCAGCAATATCATCCGCAGGCGCAGGCACATCAAGCCCCACAGCCTCATCAGCCGGCGCCACAACAGCAACTGCC
GCCAGAACAACAGGTCCCCTATCAGACACACTACCAGGAGCGGCCGCGCTATGAACCACATCCTGCAACTCAGTCCCCAA
CAGCAATACCACCATCCCAAGTCCAGCAACAATCGCATTATGCTCCTGTGGCAGAGTCCCATTATGCACCAGCCCAACAG
TCACAGTTGCCGCCGCAACAAACTACAGTACCGCAGTTGCACGCACAGCAGCCGCAGCAGCAACAAAACGGCAACGGCAA
TCCACCTCCTCAACAAAGTCCTCCGGTGACAAATGAAGTGGCTGGCCTACGCTTGCCGCGCAATTTCCACGCCTACGGGC
GCAACAACCATGAGACATGGAAGCCGGCTCTAACGCTTGAAGAGCCTGATGATCCCTCGCGCGTGTGCCACTATGCCAAG
CAGTTGGTATATCCACCAGGAGCAGGCGTCGAGTACAGTCCCGAAGAGATACTAGCCCGCAAGTTTAAGCAGTTAATGGA
TCAAAAAGCCAAGCCATCGGAGCCGCCAGAACAAGAACAGCAAACTCTTTATGATTCCTACGAAACTGAGAAATCTTACT
ATATGACTGCTGTGGATGGAGCTCTTTACGGGCAGAACACCAGCAGTGGTCAGGAGAATACAGGTGAGGAAGATGAGGAC
AATGACGCCGAGGAGGGAGAAGAGGAGGAGGATGGTGGTGAAGAGAACGAGGAAGATGACTCTGATGAAGAGGAGGAAGA
CGATGAAGAGGAAGAGGAGCATAGCGGACCGTACACAAATGGCGTGCAGTTCAGTGCGCAAACCACTTTCGAGCAAGAAA
ATCGATCGATTAAGATCAAGTTCAGGAAGGAGCCCAGCAGCACCTACAGTGCCTATACCATAGAGAATGTTTACCAGCAA
CAACAGCAGCAGCAGCAGGAGCAGCATCAAATAATCCATCAGCCTCCGCAGGCAGTACATCATCCTTCGCCGGACCCGGC
ACCTGCTTCTCCGATTCCCATCCAGCGTCAACGCAACGGAAGCCACCACCACTTCCATCCCTACATGCTGGGCCAGACCT
CGACGCCGAAGAGCGAGGCGAATGGCTATCGCAGGGCACGCACCAAAGTTAAGCGCAGCAAGTTCCAGCCGGATCTCTGC
AGCAACAGCAACTCGGCATCCTCGGTGGCTGATGTGGCCTCGAGTAGTGTGCTCGCCGGAGCACCTGGCACATTTAATGA
CAATGCAAACTTTTCGTTCTCCAGTGCCACAGCTTTGGATAACTCAAATAGCTCGCTGGCCTTGGCTGTTGATAGACTTA
ACTTCCGCGACACATCGCAACAGCAGATCCTCCATCCGGTCAATAAGACCTTACAAATTCACAACAACAACAATAACACC
AGCAACAACAATAATGGCACTAGCACCATGGCCGATTTCTCCACATTTCAAGAGAACTCGTACTTCGCCACTCAGCACGA
CACGGAGGCCCAGGAGCGTCGGCTATCGAAGGCCGTTGAAACGATAGCCCGCCACATGGACAAGGAGGCCATCGATCCGT
TCAACAGCGAACTGTGCCGCGCTTTTCTCGCCAAGCTAGATTTTCCGGGTAATCACGATGCCCATGCCAGTTACAAGATT
GTCCAAACGCCACTGCCAAAAATATCCAACACTCGCACGCTGAACGTCCTGGAAGGCGTTACCTTTAGCATCGACAAGGA
GGTGGGTCGCGGCTCATATGGCTCGGTTTATAAAGCCACTGATTCTCGGACCGGCAATGTAGTGGCGCTCAAGTATCAGA
AGCCCCCAAACACCTGGGAGATATACATATGTGATCAGGTGCTGAAACGCATCAAGGAGCCAGAGGTGTTGCCTGGCGTT
ATGGACATTTCCACGGCGATCATCGCCCCAAACGCTAGTCTAATAGCCACCGAGTTCTCACCGTTCGGTTCGCTGCTGGA
CATCAACAACAAGATCCGCCAGGCAACCACCAAAGTAATGCACGAATCGCTGGTGATGCACTTCTCCGCACAGATCTGCA
ATATCGTGGACCACCTGCATCGCCAGCACATCATCCATGCGGACATTAAACCGGACAACTTCCTGCTAATGCGTGTGCCC
AATGTGGACAGTCCGTTGCCATCGCTACGTTTGATTGACTTCGGCTGTGCTATCGATATGACCCTGTTCCCGGACGGTGA
GAAGACCAAGTTCCGGAAGGTGGTGCAAACGGATGGCTTCACCTGCATCGAGATGCAGGAGGGACGCAGCTGGTCCTACG
AAACGGATCTCTTCTGCATCGCGGCGACCGTTCATGTGATGCTCTTCGGTGACTATATGCAGCCGCAAAAGAAGGGCTCC
AGCTGGGAAATACGACAAAAGCTGCCGCGTTACCTCAAAAAGCATGTGTGGACCAAGTTCTTCGGGGATCTGCTCAACAT
GCAGGCGGACAAACTGCCAGCGCTGCATGAGATGCGTCTCATCTTCGAGGAGGAGGCGTACCGCATGGACTCCGAGCTGC
AGAAGCAGATACGCACCCTATCCAACATCCTGCATCGACGATAACCAATCTATTCCTATTCCTTTCTGTTAAATTGTTTT
GCATATAACTCTGTTGACCAAGTCACCCATTTATATTGTTTCAGTTTTTATTTTAAAACAATTTTCCCTCTTCAAAGATG
ATAAAACCAAACCGGGAAAAACTCCGCGTTCTAATTTTAAACTATTTTTCTGCGAACAAACTCCCTTTTATTTATTGTTT
TGATGCCAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: DPiM_cDNA_bait_cDNA
      Description

      Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.

      (Mintseris et al., 2009.11.23)
      Sample preparation
      Protocol

      A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.

      (Mintseris et al., 2009.11.23)
      Comment
      Reported As
      Symbol Synonyms
      DPiM_cDNA_bait
      DPiM_cDNA_bait_cDNA
      References (3)
      Research paper (2)
      Rhee et al., 2014, Cell Rep. 8(6): 2031--2043
      Transcription Factor Networks in Drosophila melanogaster. [FBrf0226298]
      Guruharsha et al., 2011, Cell 147(3): 690--703
      A Protein Complex Network of Drosophila melanogaster. [FBrf0216491]
      Personal communication to FlyBase (1)
      Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project
      DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)