FB2026_02 , released June 18, 2026
Clone: Dmel\CH322-157F4
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\CH322-157F4
Species
D. melanogaster
Name
CH322-157F4
FlyBase ID
FBcl0770236
Feature type
genomic_clone
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CHORI-322_BAC
    • Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 21kb inserts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      organism
      (Venken et al., 2009)
      Sequence Data of the Insert
      Full Length Sequence
      Genomic Location
      3R:27,207,934..27,231,390 [+]
      Total bases
      23,457
      NCBI
      Full Length sequence dataSequence Downloader
      Left End Sequence
      Total bases
      654
      NCBI
      FI425804
      Left End sequence data
      GATCCGTTGGCTTCATTTGCATTTGGCTAGAAGACAAGAGACGAGCTGCTGCTGCAGTTTCTGTGGCTCATTTAAGTGTT
AAAAATGGCAGTTACATGAAACCGAGCTGGCAAATGTTCCGTTTACGTTTACGTTTGTTTCGTGCCCATGGCTCTGTGCT
TTATGTGTTTTCCGTCTTTCCCGCTGCTGCCAGCCGAGTTCGTTGCCTGAGTCTTTTGTTTTAAATATTAAACGGGCCCT
ACAAATCGCTGCCAGCTGCCTTCCGTTCCCGATCCCGAAGCTTCTGTGGACAACTGCACAAAATTTGTCATTTTTCCCGC
GGTTTGGCGGCTCATTAGTCAGGCCGCACATTTTCGTGGAAGGCGCAGACGCAGACAGAAAGCTGCCAGGCGATGACAAG
CCAGATTAGAGACCCCAAAACGACACTGTCCCTGCTCCAGCTCCAGACCTCGATCCCTCCTCGCGTCCCTTCCCGTTCCT
GTTCTCGTTCCCACTGATATGTCCATGTACCTCGGAACTGAATCGAGTCCCAGGCGACCTAAATGTTTATTTGCTCTGCA
GACGCAAGGGCCTGACACTTTCTGTGGCGATTTTAATTAGAAGTTGGGAAATGGGAGTTTCATTTGGGCTATCAACGGGG
CTGGGCTAATAGCGATAAATTTTATCTAAATTGCATATAAAAT
      Right End Sequence
      Total bases
      699
      NCBI
      FI425803
      Right End sequence data
      GATCAGTCCACTAAAGTTAGCATGTGAAAATCGGAACGGCCTGCATTGCTCTCAACATTCCAAGGACCTGGATCGCCACT
CAAAGTCCGGATCCAGGTGCCGAAGGGAAATCAAACTGCTTAACAAGACTCTCAATTCGGGAGACCACAACTAAATGAAC
ACCATAGACAGTGTGTACCATATATAGGATGCCTTTACTCCGTAGACCAGATCCTTTTAGAGGAAAGTATACCAGATACC
AGAATTGTTTTGAATAACTTATGTATGCAACCTGTTAGCTAAATTATCTACCCAGAGTCCCCGTAAACAAAGATTATATA
TGTACGATTATATTGTAACCATACGTCGTTGTATATATGTATAGTGTGATTAAGAGGCTTCAGATGTTTATTATACATAT
GTATGTACGTATGGGATACCCACGCAATCGCTTATATTGTAATTATACACTCAGTCTAGTCAAATACAAATAATAATATT
ATTGTAATTATATACTATGAATAAATATCGAGACTATATGAATCTACAAATCCATGCAATGTACTATACGCGTTGGAACT
TCTGCATATATTCTAATCCACGTACTCCTCCAAATCGAACCCAGAGAAATAAAACTTATCCGAACCTCAAGTCTTTCATT
TTCCACCATACTAACATCGAGATTAAAGTTAACGACGACAGATTACAACAACGTTGGTA
      Library Information (1)
      Library: CHORI-322_BAC
      Description

      A BAC library containing genomic DNA fragments (average size of 21 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

      (Venken et al., 2009)
      Sample preparation
      Protocol

      DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 20-25 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

      (Venken et al., 2009)
      Comment

      A total of 73,728 clones were generated.

      (Venken et al., 2009)
      Reported As
      Symbol Synonyms
      CHORI-322
      (Venken et al., 2009)
      CHORI-322_BAC
      References (3)
      Research paper (2)
      Venken et al., 2010, Genetics 186(4): 1111--1125
      A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
      Venken et al., 2009, Nat. Methods 6(6): 431--434
      Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. [FBrf0209231]
      Personal communication to FlyBase (1)
      GenetiVision Corporation, 2016-, A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome.
      A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome. [FBrf0232631]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      PACMANFLY - The source for information and resources of tools for the Drosophila community using the P[acman] recombineering technique.
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CH322-157F4
      Secondary FlyBase IDs
        References (1)