FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Split System Combination: Scer\GAL4DBD.R17C11∩Hsap\RELAAD.R52G04
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General Information
Symbol
Scer\GAL4DBD.R17C11∩Hsap\RELAAD.R52G04
FlyBase ID
FBco0000019
Feature Type
split system combination
Component Alleles
Combination components
Component allele Scer\GAL4DBD.R17C11
Component
Use(s)
Regulatory region(s)
Description

The R17C11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Component allele Hsap\RELAAD.R52G04
Component
Use(s)
Regulatory region(s)
Description

The R52G04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Expression Data
Reporters Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In a split-GAL4 system, ScerGAL4DBD.R17C11 drives expression in mushroom body output neuron 7 when combined with HsapRELAAD.R52G04.

In a split-GAL4 system, HsapRELAAD.R52G04 drives expression in 1-3 medulla columnar neuron VPN-MB2 as well as mushroom body output neuron 7 when combined with ScerGAL4DBD.R49F03 and in mushroom body output neuron 7 when combined with ScerGAL4DBD.R17C11. Expression overlaps with that driven by EcollexAGMR13F02 in the Kenyon cells. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between medulla columnar neuron VPN-MB2 and gamma dorsal Kenyon cells using these split-GAL4 drivers and AvicGFPspGFP11.Mef2.mb247.T:HsapCD4.

Marker for
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)