FlyBase Split System Combination Report: Scer\GAL4[DBD.R14C08]∩Hsap\RELA[AD.R12C11]

General Information

Symbol

Scer\GAL4^DBD.R14C08∩Hsap\RELA^AD.R12C11

FlyBase ID

FBco0000052

Feature Type

split system combination

Component Alleles

Scer\GAL4^DBD.R14C08

Hsap\RELA^AD.R12C11

Combination components

Component allele Scer\GAL4^DBD.R14C08

Component

Use(s)

Regulatory region(s)

GMR14C08

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The R14C08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Component allele Hsap\RELA^AD.R12C11

Component

Use(s)

Regulatory region(s)

GMR12C11

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R12C11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body output neuron 1

(Scaplen et al., 2021, Lewis et al., 2015, Aso et al., 2014)

mushroom body output neuron 3

(Lei et al., 2022, Scaplen et al., 2021, Dolan et al., 2018, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)