The R26A03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R54A05 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R26A03 drives expression in lobula columnar neuron LC15 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R24A02, and lobula columnar neuron LC16 when combined with ScerGAL4DBD.R54A05.
In a split-GAL4 system, ScerGAL4DBD.R54A05 drives expression in lobula columnar neuron LC16 when combined with HsapRELAAD.R26A03, or LC16 and a subset of neurons in the thoracico-abdominal ganglion when combined with HsapRELAAD.R82D11.