FlyBase Split System Combination Report: Scer\GAL4[DBD.R35D04]∩Hsap\RELA[AD.R47E07]

General Information

Symbol

Scer\GAL4^DBD.R35D04∩Hsap\RELA^AD.R47E07

FlyBase ID

FBco0000264

Feature Type

split system combination

Component Alleles

Hsap\RELA^AD.R47E07

Scer\GAL4^DBD.R35D04

Combination components

Component allele Hsap\RELA^AD.R47E07

Component

Use(s)

Regulatory region(s)

GMR47E07

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R47E07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)

Component allele Scer\GAL4^DBD.R35D04

Component

Use(s)

Regulatory region(s)

GMR35D04

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Description

The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)

Expression Data

Reporters Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lobula columnar neuron LC10b

(Wu et al., 2016)

lobula columnar neuron LC10d

(Wu et al., 2016)

lobula columnar neuron LC10c

(Wu et al., 2016)

Additional Information

Statement

Reference

In a split-GAL4 system, HsapRELA^AD.R47E07, inserted in P{R47E07-p65.AD}, drives expression in lobula columnar neuron Lcn6 when combined with ScerGAL4^DBD.R41C07, Lcn6 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4^DBD.VT049344, lobula columnar neuron LC10b and lobula columnar neuron LC10d when combined with ScerGAL4^DBD.R35D04 and, when inserted in PBac{R47E07-p65.AD}, lobula columnar neuron LC10c when combined with ScerGAL4^DBD.R35D04.

(Wu et al., 2016)

In a split-GAL4 system, ScerGAL4^DBD.R35D04 drives expression in lobula columnar neuron LC10b when combined with HsapRELA^AD.R22A07, lobula columnar neuron LC10b and lobula columnar neuron LC10d when combined with HsapRELA^AD.R47E07 inserted in P{R47E07-p65.AD}, and lobula columnar neuron LC10c when combined with HsapRELA^AD.R47E07 inserted in PBac{R47E07-p65.AD}.

(Wu et al., 2016)

Marker for

Stocks (2)

Bloomington

68377

w¹¹¹⁸; P{R47E07-p65.AD}attP40; P{R35D04-GAL4.DBD}attP2

68379

w¹¹¹⁸; P{R35D04-GAL4.DBD}attP2 PBac{R47E07-p65.AD}VK00027

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym