The R55C04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R85F11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R55C04 drives expression in lobula columnar neuron LC21 when combined with ScerGAL4DBD.R41C05, and lobula columnar neuron LC21 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R85F11.
In a split-GAL4 system, ScerGAL4DBD.R85F11 drives expression in lobula columnar neuron LC21 and a subset of neurons in the thoracico-abdominal ganglion when combined with HsapRELAAD.R55C04.