The R22A07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R22A07 drives expression in lobula columnar neuron LC10b when combined with ScerGAL4DBD.R35D04.
In a split-GAL4 system, ScerGAL4DBD.R35D04 drives expression in lobula columnar neuron LC10b when combined with HsapRELAAD.R22A07, lobula columnar neuron LC10b and lobula columnar neuron LC10d when combined with HsapRELAAD.R47E07 inserted in P{R47E07-p65.AD}, and lobula columnar neuron LC10c when combined with HsapRELAAD.R47E07 inserted in PBac{R47E07-p65.AD}.