The R92B02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
The R38E08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, HsapRELAAD.R92B02 in the P{R92B02-p65.AD} construct drives expression in lobula columnar neuron Lcn6 when combined with ScerGAL4DBD.R41C07 and, when inserted into PBac{R92B02-p65.AD}, in lobula columnar neuron LC16 and a subset of neurons in the thoracico-abdominal ganglion when combined with ScerGAL4DBD.R38E08.
In a split-GAL4 system, ScerGAL4DBD.R38E08 drives expression in lobula columnar neuron LC16 and a subset of neurons in the thoracico-abdominal ganglion when combined with HsapRELAAD.R92B02 inserted into PBac{R92B02-p65.AD} in a split-GAL4 system.