The R94D12 enhancer fragment and Drosophila synthetic core promoter (DSCP) drive expression the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R67E03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
In a split-GAL4 system, ScerGAL4DBD.R94D12 drives expression in lobula columnar neuron LC19 when combined with HsapRELAAD.R67E03.