The R66A01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R48A08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Employing the intersectional Split-Gal4 method ScerGAL4DBD.R66A01 drives expression exclusively in around 60% of lamina monopolar neurons L1 when combined with HsapRELAAD.R48A08.
Labels around 60% of lamina monopolar neurons L1.