The ple promoter region is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R24E12 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
In a Split-GAL4 system, HsapRELAAD.R24E12 drives expression 10 dopaminergic PAM neurons when combined with ScerGAL4DBD.ple.
Labels 10 dopaminergic PAM neurons.