The R48D11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R25B02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Employing the intersectional Split-Gal4 method ScerGAL4DBD.R48D11 drives expression in centrifugal neurons C2, C3 and in less than 10% of columnar neurons T1 when combined with HsapRELAAD.R20C11. In combination with HsapRELAAD.R25B02 expression is driven exclusively in centrifugal neurons C2.