The R75H07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R59A05 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Employing the intersectional Split-Gal4 method ScerGAL4DBD.R75H07 drives expression in lamina monopolar neurons L3 and in around 20% of lamina wide-field cells Lawf2 when combined with HsapRELAAD.R59A05 .
Labels around 20% of lamina wide-field cells Lawf2.