The R17D06 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R92A10 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Employing the intersectional Split-Gal4 method ScerGAL4DBD.R17D06 drives expression in lamina intrinsic neurons and in less than 20 transmedullary neurons which project to the adult cerebrum when combined with HsapRELAAD.R92A10.
Labels less than 20 transmedullary neurons which project to the adult cerebrum.