The R66A02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
The R92A10 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Employing the intersectional Split-Gal4 method ScerGAL4DBD.R66A02 drives expression exclusively in over 80% of lamina intrinsic neurons when combined with HsapRELAAD.R92A10.
Labels over 80% of lamina intrinsic neurons.