Consists of ort promoter sequences containing conserved regions C1-C3, the hs43 mini-promoter and the lexA(DBD)::Zip- hemidriver (component of a 'split driver' system, contains the lexA DNA-binding domain).
The ort C2b enhancer and hs43 mini-promoter regulate expression of a VP16(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the VP16 activation domain).
In a split-GAL4 system, EcollexADBD.C1-3.ort drives expression mainly in distal medullary amacrine neuron Dm8 when combined with HsimVP16AD.ort.C2b.