Insertion of P{ET.VP16.AD}.
P{ET.VP16.AD} is inserted into the first intron of tou.
The ort C1 enhancer followed by the hs43 minimal promoter regulate expression of a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsimVP16AD.tou-9A30 drives expression mainly in transmedullary neuron Tm20 when combined with ScerGAL4DBD.C1a.ort.