A tubulin promoter drives expression of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
2A-p65(AD)::Zip+ is introduced into the attP site in the TI{TI}unc-4FRT progenitor. The Trojan insertion introduces a stop codon, truncating the unc-4 protein after its first exon.