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| Term | polyA trap | ID (Ontology) | FBcv:0005072 (FlyBase CV) | ||||||||
| Definition | The basic components of a polyA trap cassette are a promoter fused to a gene sequence that lacks a polyadenylation (polyA) sequence but is followed by a splice donor sequence. If the cassette is integrated into the genome outside a gene, the mRNA produced by the polyA trap is not polyadenylated and is expected to be rapidly degraded. However, if the cassette is integrated into an intron, the promoter in the polyA trap will drive expression of a transcriptional fusion containing the polyA gene trap fused to any downstream exons. The downstream terminal exon can provide a polyA signal, allowing stable expression of the gene encoded by the polyA trap. polyA trap cassettes can be used to report the expression of genes that are not normally expressed, or are expressed at very low levels, or may be used as a marker of successful integration of an insertion into a transcription unit. A polyA trap cassette may be inserted into a genome as part of a transgenic construct via transposable-element-mediated transgenesis, or may be inserted directly into a modified endogenous locus via a genome engineering method such as homologous recombination or CRISPR.[ FlyBase:FBrf0132344 FlyBase:FBrf0206814 PubMed:9560157 ] | ||||||||||
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experimental tool descriptor |__insertional mutagenesis tool |__polyA trap 7 rec. |
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| Is a | insertional mutagenesis tool | ||
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