amy1, Amy, Amy 1
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Genomic restriction maps were generated of the Amy region. Polymorphic variants were tested in pairwise combinations and strong linkage disequilibrium was found among the gene arrangements.
The functional locus is in chromosome X2, at a site homologous to section 73A of its sibling species. A duplicated copy is also present but fails to produce a functional product in transient somatic expression assays. In situ hybridization studies suggest that two other sites in the X2 chromosome might contain inactive sequences belonging to the Amy gene family.
Transient expression assays were used in somatic transformation experiments to functionally test the status of the Amy genes in D.melanogaster embryos.
Amylase activity is repressed by the presence of glucose in the diet. Dosage compensation for expression occurs in male larvae whether they are reared on a repressive diet containing glucose or on a nonrepressive diet without it.
