Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
A Dsim\Hmr+ transgene has no phenotypic effect in D.melanogaster/D.simulans and D.melanogaster/D.mauritiana hybrids and a Dmau\Hmr+ transgene has no phenotypic effect in D.melanogaster/D.simulans and D.melanogaster/D.mauritiana hybrids, strongly supporting the conclusion that the Hmr gene has functionally diverged between the D.melanogaster and sibling-species (D.simulans and D.mauritiana) lineages, to cause F1 hybrid incompatibility between these species. Phylogenetic analysis shows that Hmr has diverged extensively in the D.melanogaster lineage, but extensive divergence has also been found in the sibling-species lineage. Together, the findings implicate over 13% of the amino acids encoded by Hmr as candidates for causing hybrid incompatibility. The exceptional level of divergence at Hmr cannot be explained by neutral processes, as phylogenetic methods and population genetic analyses show that the elevated amino-acid divergence in both lineages is due to positive selection in the distant past - at least one million generations ago.
