FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
galla2, Ciao1
FlyBase ID
FBig0000031493
Interaction Network
Interactions Browser links
galla2 network
Ciao1 network
Reported Interactions
FBrf0218395-6030.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ciao1 
unspecified role
Ciao1 
unspecified role
Ciao1 
unspecified role
galla2 
unspecified role
galla2 
unspecified role
galla2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 89.43362

FBrf0245667-5.PD.WB
Reference
Jung et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
galla2 

Galla2

bait

GST tag

Ciao1 

Ciao1

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Jung et al., 2020)

Weak interaction.

(Jung et al., 2020)
FBrf0247217-1.XC
Reference
Kassube and Thomä, 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ciao1 

CIAO1

neutral component
Ciao1 

CIAO1

neutral component
Ciao1 

CIAO1

neutral component
galla2 

CIAO2B

neutral component
galla2 

CIAO2B

neutral component
galla2 

CIAO2B

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

(Kassube and Thomä, 2020)
FBrf0247217-2.PD.MW
Reference
Kassube and Thomä, 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
galla2 

CIAO2B

bait

Strep tag

Ciao1 

CIAO1

prey

His tag

Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ciao1 
interaction domain residue 18
mutation decreasing interaction
aa 18

W18A

Ciao1 
interaction domain residue 63
mutation decreasing interaction
aa 63

R63E

Ciao1 
interaction domain residue 79
mutation decreasing interaction
aa 79

F79A

Ciao1 
interaction domain residue 107
mutation decreasing interaction
aa 107

K107E

Ciao1 
interaction domain residue 152
mutation decreasing interaction
aa 152

K152E

Ciao1 
interaction domain residue 168
mutation decreasing interaction
aa 168

Y168A

Ciao1 
interaction domain residue 305
mutation decreasing interaction
aa 305

D305K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

(Kassube and Thomä, 2020)
FBrf0247217-3.PD.MW
Reference
Kassube and Thomä, 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ciao1 

CIAO1

bait

His tag

Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
Ciao1 
galla2 

CIAO2B

prey

Strep tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ciao1 
interaction domain residue 18
mutation decreasing interaction
aa 18

W18A

Ciao1 
interaction domain residue 63
mutation decreasing interaction
aa 63

R63E

Ciao1 
interaction domain residue 79
mutation decreasing interaction
aa 79

F79A

Ciao1 
interaction domain residue 107
mutation decreasing interaction
aa 107

K107E

Ciao1 
interaction domain residue 152
mutation decreasing interaction
aa 152

K152E

Ciao1 
interaction domain residue 168
mutation decreasing interaction
aa 168

Y168A

Ciao1 
interaction domain residue 305
mutation decreasing interaction
aa 305

D305K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.

(Kassube and Thomä, 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)