Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovarian cDNA expression library).

Source was adult heads of wild-type or comt⁴ temperature sensitive fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Immunoprecipitation of αSnap was more efficient in comt⁴ temperature sensitive mutant background than in wild-type flies.

Flies were exposed to 38oC for 10min for tissue collection and preparation of head homogenates.