FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4E1, mxt
FlyBase ID
FBig0000044028
Interaction Network
Interactions Browser links
eIF4E1 network
mxt network
Reported Interactions
FBrf0222078-10.FW.A
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

4E1

bait

[32]P label

mxt 

Mxt

prey
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
eIF4E-binding motif
mutation disrupting interaction
YDIEHLL

\'Mxt[AAA]\', Y581A, L586A, L587A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Hernández et al., 2013)

Four clones corresponding to CG2950 were identified.

(Hernández et al., 2013)
FBrf0222078-11.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mxt 

Mxt

bait

HA tag

mxt 
mxt 
eIF4E1 

eIF4E-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
eIF4E-binding motif
mutation disrupting interaction
YDIEHLL

\'Mxt[AAA]\', Y581A, L586A, L587A

mxt 
C-terminal region
sufficient binding region
aa 561-653
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Hernández et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hernández et al., 2013)

CG2950 (mxt) binds eIF-4E, eIF3-S8, Int6, eIF-3p40 and CG3225, but not eIF-4a, pAbp, eIF4G, eIF4AIII or Cbp80 in this assay.

(Hernández et al., 2013)

This interaction persists in the presence of RNaseA.

(Hernández et al., 2013)
FBrf0222078-16.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

4E1

bait
mxt 

Mxt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction persists in the presence of RNaseA.

(Hernández et al., 2013)

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hernández et al., 2013)
FBrf0222078-17.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mxt 

Mxt

bait
eIF4E1 

4E1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction persists in the presence of RNaseA.

(Hernández et al., 2013)

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hernández et al., 2013)
FBrf0222078-19.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mxt 

Mxt

bait

V5 tag

mxt 
eIF4E1 

4E1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
eIF4E-binding motif
mutation disrupting interaction
YDIEHLL

\'Mxt[AAA]\', Y581A, L586A, L587A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hernández et al., 2013)

This interaction persists in the presence of RNaseA.

(Hernández et al., 2013)
FBrf0222078-2.FW.A
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 17
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1

bait

[32]P label

mxt 

CG2950

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Four clones corresponding to CG2950 were identified.

(Hernández et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Hernández et al., 2013)
FBrf0222078-3.Y2H
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1, eIF4E-2

bait

fused to GAL4 DNA-binding domain

mxt 

Mxt

prey

fused to GAL4 activation domain

mxt 
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
C-terminal region
sufficient binding region
aa 284-653
mxt 
eIF4E-binding motif
mutation disrupting interaction
YDIEHLL

\'Mxt[AAA]\', Y581A, L586A, L587A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hernández et al., 2013)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hernández et al., 2013)

CG2950 (mxt) binds eIF4E, eIF4E-3, eIF4E-4 and eIF4E-7, but not eIF4E-6 or 4EHP in this assay.

(Hernández et al., 2013)
FBrf0222078-7.coIP.WB
Reference
Hernández et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E-1

bait

HA tag

mxt 

Mxt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CG2950 (mxt) binds eIF-4E but not eIF-4E-6 or 4EHP in this assay.

(Hernández et al., 2013)

CG2950 (mxt) exhibited the strongest affinity for eIF-4E, compared to eIF4G and Thor.

(Hernández et al., 2013)

This interaction persists in the presence of RNaseA.

(Hernández et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hernández et al., 2013)
FBrf0229498-1.coIP.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

mxt 

Mxt

prey

GFP tag

mxt 
mxt 
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
alpha 1 domain
necessary binding region
aa 581-592
mxt 
alpha 2 domain
necessary binding region
aa 602-608
mxt 
alpha 3 domain
necessary binding region
aa 627-636
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peter et al., 2015)
FBrf0229498-2.PD.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

mxt 

Mxt

prey

GFP tag

mxt 
mxt 
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
alpha 1 domain
necessary binding region
aa 581-592
mxt 
alpha 2 domain
necessary binding region
aa 602-608
mxt 
alpha 3 domain
necessary binding region
aa 627-636
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cells.

(Peter et al., 2015)
FBrf0229498-3.PD.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

His6 tag

mxt 

Mxt

prey

MBP tag

mxt 
mxt 
mxt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mxt 
alpha 1 domain
necessary binding region
aa 581-592
mxt 
alpha 2 domain
necessary binding region
aa 602-608
mxt 
alpha 3 domain
necessary binding region
aa 627-636
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived recombinant fusion protein in bacterial system.

(Peter et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)