FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dan, ey
FlyBase ID
FBig0000086268
Interaction Network
Interactions Browser links
dan network
ey network
Reported Interactions
FBrf0201164-10.Y2H
Reference
Curtiss et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dan 

Dan

bait

fused to GAL4 DNA-binding domain

ey 

Ey

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Curtiss et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Curtiss et al., 2007)
FBrf0201164-13.Y2H
Reference
Curtiss et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ey 

Ey

bait

fused to GAL4 DNA-binding domain

dan 

Dan

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Curtiss et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Curtiss et al., 2007)
FBrf0201164-2.PD.A
Reference
Curtiss et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dan 

Dan

bait

GST tag

ey 

Ey

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Curtiss et al., 2007)
FBrf0211770-10.coIP.WB
Reference
Hoang et al., 2010
Description
physical association
Assay
Collection
Source/Stage
eye-antennal disc / third instar larval stagelarval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ey 

Ey

bait

FLAG tag

dan 

Dan

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0211770-9.coIP.WB
Reference
Hoang et al., 2010
Description
physical association
Assay
Collection
Source/Stage
eye-antennal disc / third instar larval stagelarval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dan 

Dan

bait
ey 

Ey

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)