FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bcd, Bin1
FlyBase ID
FBig0000094918
Interaction Network
Interactions Browser links
bcd network
Bin1 network
Reported Interactions
FBrf0126798-2.Y2H
Reference
Zhu and Hanes, 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

Bicoid

bait

bcd DNA-binding domain

bcd 
Bin1 

dSAP18

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
DNA-binding domain
unspecified role
aa 1-343
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from embryonic cDNA library.

(Zhu and Hanes, 2000)

Two-hybrid system: a custom yeast system that uses bcd-response elements and the bcd DNA-binding domain.

(Zhu and Hanes, 2000)
FBrf0136855-1.Y2H
Reference
Zhu et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Bin1 

Bin1/dSAP18

bait

fused to LexA DNA-binding domain

bcd 

Bicoid

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhu et al., 2001)

Two-hybrid system: yeast LexA-BD/B42-AD

(Zhu et al., 2001)
FBrf0136855-2.Y2H
Reference
Zhu et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

Bicoid

bait

fused to LexA DNA-binding domain

bcd 
bcd 
Bin1 

Bin1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
N-terminal region
sufficient binding region
aa 1-95

coordinates relative to bcd-PD

bcd 
central region
sufficient binding region
aa 136-246

coordinates relative to bcd-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhu et al., 2001)

Two-hybrid system: yeast LexA-BD/B42-AD

(Zhu et al., 2001)
FBrf0136855-3.PD.A
Reference
Zhu et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Bin1 

Bin1

bait

GST tag

bcd 

Bcd

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Zhu et al., 2001)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)