Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

U2af50 and U2af38 form a U2AF heterodimer.

Both proteins were coexpressed in E. coli cells.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The U2af50 constructs tested for interaction with endogenous U2af38 rescue the U2af50 mutant.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

U2af50 and U2af38 form a U2AF heterodimer.

Both proteins were coexpressed in E. coli cells.