SXL
fused to LexA DNA-binding domain
SIN
fused to B42 activation domain
Two-hybrid system: yeast LexA/B42.
GST tag
[35]S label
sufficient for weak interaction
Bait expressed and purified from bacteria.
Prey derived from in vitro translation.
Sin binds to the Sxl RBD1 domain with ten-fold greater affinity than to the Sxl RBD2 domain.
The interaction of Sxl and Sin proteins is resistant to treatment with RNaseA and RNaseT1.
The interaction of Sxl and Sin proteins is resistant to 1M NaCl.
