Source was a chromatin extract from embryos derived from transgenic line carrying tagged cav construct.
Source was extract from embryos derived from transgenic line carrying tagged cav construct.
Embryonic extracts from transgenic line carrying tagged cav construct.
Su(var)205, cav, moi and ver were co-expressed in bacteria. This was supplemented with purified HipHop, and proteins were separated by heparin-sepharose then size-exclusion chromatography to identify complexes formed.
ver-moi and Su(var)205-cav-HipHop form separate subcomplexes in vitro. All five proteins were not observed to form a single "terminin" complex in vitro.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
