Su(var)205, cav, moi and ver were co-expressed in bacteria. This was supplemented with purified HipHop, and proteins were separated by heparin-sepharose then size-exclusion chromatography to identify complexes formed.

ver-moi and Su(var)205-cav-HipHop form separate subcomplexes in vitro. All five proteins were not observed to form a single "terminin" complex in vitro.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.