Bait derived from in vitro translation.

Prey derived from in vitro translation.

[35]S labeled bait and prey distinguished by molecular weight.

mira protein binds to stau protein through the RBD5 domain, but not its RBD1 domain.

Two-hybrid system: yeast LexA/VP16.

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

GST pull down.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Kd = 1.1 uM

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

A 2:2 heterotetramer was observed, in which two mira coiled-coils form a homodimer, with one stau RBD5 subunit bound to the N-terminal end of each mira coiled-coil.