FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
bcd, bin3
FlyBase ID
FBig0000095548
Interaction Network
Interactions Browser links
bcd network
bin3 network
Reported Interactions
FBrf0126798-1.Y2H
Reference
Zhu and Hanes, 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

Bicoid

bait

bcd DNA-binding domain

bcd 
bin3 

Bin3

prey

fused to B42 activation domain

bin3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
DNA-binding domain
unspecified role
aa 1-343
bin3 
region
unspecified role
aa 61-1368
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from embryonic cDNA library.

(Zhu and Hanes, 2000)

Two-hybrid system: a custom yeast system that uses bcd-response elements and the bcd DNA-binding domain.

(Zhu and Hanes, 2000)
FBrf0126798-3.Y2H
Reference
Zhu and Hanes, 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

Bicoid

bait

fused to LexA DNA-binding domain

bcd 
bin3 

Bin3

prey

fused to B42 activation domain

bin3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
region
unspecified role
aa 3-489
bin3 
region
unspecified role
aa 61-948
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA/B42.

(Zhu and Hanes, 2000)
FBrf0126798-4.PD.A
Reference
Zhu and Hanes, 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bin3 

Bin3

bait

GST tag

bin3 
bcd 

Bcd

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bin3 
region
unspecified role
aa 132-948
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Zhu and Hanes, 2000)

Prey derived from in vitro translation.

(Zhu and Hanes, 2000)

GST pull down.

(Zhu and Hanes, 2000)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)