FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ebd1, pygo
FlyBase ID
FBig0000095582
Interaction Network
Interactions Browser links
ebd1 network
pygo network
Reported Interactions
FBrf0213544-10.coIP.WB
Reference
Benchabane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ebd1 

Ebd1

bait

GST tag

pygo 

Pygo

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Benchabane et al., 2011)

Bait expressed and purified from bacteria.

(Benchabane et al., 2011)

Prey expressed in transfected HEK293T cells.

(Benchabane et al., 2011)
FBrf0213544-2.PD.WB
Reference
Benchabane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

Pygo

bait

GST tag

ebd1 

Ebd1

prey

His tag, V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Benchabane et al., 2011)

Bait expressed and purified from bacteria.

(Benchabane et al., 2011)

Prey expressed and purified from bacteria.

(Benchabane et al., 2011)
FBrf0213544-6.PD.WB
Reference
Benchabane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ebd1 

Ebd1

bait

GST tag

ebd1 
pygo 

Pygo

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ebd1 
CENPB domain
unspecified role
aa 188

necessary: the ebd1[QF1] I188F mutation abolishes interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST pull down.

(Benchabane et al., 2011)

Bait expressed and purified from bacteria.

(Benchabane et al., 2011)

Prey expressed in transfected HEK293T cells.

(Benchabane et al., 2011)
FBrf0213544-9.coIP.WB
Reference
Benchabane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ebd1 

Ebd1

bait

FLAG tag

pygo 

Pygo

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK293T cells.

(Benchabane et al., 2011)

Prey expressed in transfected HEK293T cells.

(Benchabane et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)