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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095666
Interaction Network
Interactions Browser links
lin-52 network
Myb network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180259-27.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

Lin-52

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.

FBrf0200827-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Lin52

bait
Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0219063-12.CH.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Lin-52

bait

FLAG tag

Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.

Prey endogenous to embryonic nuclear extract and coimmunoprecipitated with FLAG-tagged lin-52.

Myb associates with lin-52 as part of the MMB/dREAM complex.

lin-52 fractionates exclusively with components of the MMB/dREAM complex.

Proteins associated with affinity purified FLAG-tagged lin-52 were subjected to gel filtration.

FBrf0219063-5.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Lin-52

bait

FLAG tag

Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.

Prey endogenous to embryonic nuclear extract.

FBrf0245601-2.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

LIN52

bait
Myb 

dMyb

prey
Myb 
Myb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myb 
interaction domain
mutation decreasing interaction
aa 606,607

K606A,W607A

Myb 
interaction domain
mutation disrupting interaction
aa 617,618

D617A,Q618A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding of Myb to a mip130-lin-52 heterodimer was captured as two binary interactions.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)