Scr is tagged with N- or C-terminal portions of Venus fluorescent protein that fluoresce when two Scr molecules containing complementary fragments interact.

Bait expressed in transfected HEK 293 T cells.

Prey expressed in transfected HEK 293 T cells.

To test for homodimerization, V5-tagged Scr was affinity captured and probed for coimmunoprecipitation of FLAG-tagged Scr.

Mutation of Thr and Ser residues to Asp phosphomimics the inactive state of Scr.

Bait expressed in transfected HEK 293 T cells.

Prey expressed in transfected HEK 293 T cells.

Mutation of Thr and Ser residues in the homeodomain weakens homodimerization of minimal Scr constructs, but not full length Scr.

Scr is tagged with N- or C-terminal portions of Venus fluorescent protein that fluoresce when two Scr molecules containing complementary fragments interact.

Mutation of Thr and Ser residues to Asp phosphomimics the inactive state of Scr.

Proteins derived from a transgenic line carrying Venus-tagged UAS-Scr constructs.

Portions of Antp, which does not homodimerize, were replaced with corresponding portions of Scr, which does homodimerize, to map regions of Scr that mediate homodimerization.

To test for homodimerization, V5-tagged Antp-Scr chimeric protein was affinity captured and probed for coimmunoprecipitation of FLAG-tagged Antp-Scr chimeric protein.

Bait expressed in transfected HEK 293 T cells.

Prey expressed in transfected HEK 293 T cells.

The Scr linker region (between YPWM motif and homeodomain) can interact with the Scr C-terminus.