FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
PolH, Rev1
FlyBase ID
FBig0000095751
Interaction Network
Interactions Browser links
PolH network
Rev1 network
Reported Interactions
FBrf0204714-1.Y2H
Reference
Kosarek et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rev1 

Rev1

bait

fused to GAL4 activation domain

Rev1 
PolH 

Polη

prey

fused to GAL4 DNA-binding domain

PolH 
PolH 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rev1 
C-terminal domain
sufficient binding region
aa 878-995
PolH 
N-terminal region
sufficient binding region
aa 1-280
PolH 
C-terminal region
sufficient binding region
aa 521-624
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

DNApol-η can interact with Rev1 via two independent regions.

(Kosarek et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Kosarek et al., 2008)
FBrf0204714-4.coIP.WB
Reference
Kosarek et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PolH 

Polη

bait

YFP tag

Rev1 

Rev1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Kosarek et al., 2008)

Prey expressed in transfected cells.

(Kosarek et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)