FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095753
Interaction Network
Interactions Browser links
ppk29 network
ppk25 network
Reported Interactions
FBrf0219294-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ppk25

bait

FLAG tag

Nope

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK293 cells.

Prey expressed in transfected HEK293 cells.

The interaction of ppk29 (nope) with ppk25 is not observed when the two DEG/ENaC subunits are expressed in separate cells and the cellular extracts then mixed.

FBrf0219294-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nope

bait

FLAG tag

Ppk25

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK293 cells.

Prey expressed in transfected HEK293 cells.

The interaction of ppk29 (nope) with ppk25 is not observed when the two DEG/ENaC subunits are expressed in separate cells and the cellular extracts then mixed.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)