Bait endogenous to cell extract.

Prey endogenous to cell extract.

Bait expressed and purified from bacteria.

Prey derived from in vitro transcription.

Source was adult wild-type flies; bait produced from endogneous gene; prey produced from endogenous gene.

Positive control

Three-hybrid system: yeast LexA-BD fused to MCP coat protein/GAL4-AD/ snRNA:7SK fused to RNA MCP-binding RNA sequence

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).