Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type ovary extract.

GRNA chromatography: grk RNA containing a BoxB sequence of the λ phage was bound to a fusion of GST with the 21 residue N antiterminator peptide.

The grk-GST particles were pre-bound to resin and incubated with ovary extract.

Source was adult ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

In this assay, no interaction was detected between Syp and another localized RNA, bcd.

Syp interacts with grk and osk RNA, but not bcd, nos or hb RNA.

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.