FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cnb, ana2
FlyBase ID
FBig0000096880
Interaction Network
Interactions Browser links
Cnb network
ana2 network
Reported Interactions
FBrf0220918-17.Y2H
Reference
Januschke et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cnb 

CNB

bait

fused to GAL4 DNA-binding domain

ana2 

Ana2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Januschke et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Januschke et al., 2013)
FBrf0220918-4.coIP.MS
Reference
Januschke et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cnb 

CNB

bait

LAP tag

ana2 

Ana-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Januschke et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)